Interactions of human serum albumin with bioactive 3H-imidazo[4,5-a]acridines: Insights from fluorescence spectroscopic studies
- Авторы: Rahbari M.1, Pordel M.1, Chamani J.2
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Учреждения:
- Department of Chemistry, Mashhad Branch
- Department of Biochemistry and Biophysics, Faculty of Sciences, Mashhad Branch
- Выпуск: Том 42, № 1 (2016)
- Страницы: 36-41
- Раздел: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227825
- DOI: https://doi.org/10.1134/S1068162016010131
- ID: 227825
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Аннотация
Several 3H-imidazo[4,5-a]acridine derivatives were conveniently synthesized by the reaction of imidazo[4,5-a]acridones in boiling POCl3. The imidazoacridones were obtained by rearrangement of 3H-imidazo[4',5':3,4]benzo[c]isoxazoles in concentrated sulfuric acid containing nitrous acid at room temperature. The structures of all newly synthesized compounds were confirmed by IR, 1H NMR, and mass spectral data. The interactions of 3H-imidazo[4,5-a]acridines with human serum albumin (HSA) were studied by fluorescence spectroscopy. The binding of 3H-imidazo[4,5-a]acridines quenches the HSA fluorescence, revealing a 1: 1 interaction with a binding constant of about 2.34 × 105–3.16 × 106 M–1. A decrease in fluorescence intensity at 339 nm, when excited at 280 nm, is attributed to changes in the environment of the protein fluorophores caused by the presence of the ligand. The differences in interactions of 3H-imidazo[4,5-a]acridines with HSA were observed using spectrofluorimetry technique.
Об авторах
Maryam Rahbari
Department of Chemistry, Mashhad Branch
Email: mehdipordel58@mshdiau.ac.ir
Иран, Mashhad
Mehdi Pordel
Department of Chemistry, Mashhad Branch
Автор, ответственный за переписку.
Email: mehdipordel58@mshdiau.ac.ir
Иран, Mashhad
Jamshidkhan Chamani
Department of Biochemistry and Biophysics, Faculty of Sciences, Mashhad Branch
Email: mehdipordel58@mshdiau.ac.ir
Иран, Mashhad