Physicochemical Properties of the Phosphoryl Guanidine Oligodeoxyribonucleotide Analogs
- 作者: Pyshnyi D.1,2, Dyudeeva E.1, Kupryushkin M.1, Lomzov A.1,2, Pyshnaya I.1
-
隶属关系:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Novosibirsk State University
- 期: 卷 45, 编号 6 (2019)
- 页面: 709-718
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229309
- DOI: https://doi.org/10.1134/S1068162019060153
- ID: 229309
如何引用文章
详细
This work describes the basic physicochemical properties of phosphoryl guanidine oligonucleotides (PGOs)—a new type of DNA analog with a partially or totally uncharged backbone. Replacement of the negatively charged oxygen atom with an electroneutral 1,3-dimethylimidazolidine-2-imine (DMI) group is shown to influence the hydrophobicity of oligonucleotide, its electrophoretic mobility, and UV absorption spectra. Thermal stability of DMI-containing DNA-duplexes was studied in aqueous solutions with different ionic strength. It is determined that, in the case of low ionic strength, the presence of DMI groups significantly enhances the thermal stability of the duplex. In the case of nearly physiological condition (0.1 M NaCl, 10 mM MgCl2) or higher ionic strength, DMI groups do not affect the duplex stability or even slightly reduce it (melting temperature changes, on average, by –1.2°C per one DMI group). An important feature of totally uncharged PGO is its ability to form the duplex effectively regardless the ionic strength, even in deionized water. In addition, according to CD data, DMI groups do not disturb the double helix structure of DNA, maintaining it in the B form.
作者简介
D. Pyshnyi
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Novosibirsk State University
编辑信件的主要联系方式.
Email: pyshnyi@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
E. Dyudeeva
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: pyshnyi@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
M. Kupryushkin
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: pyshnyi@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Lomzov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Novosibirsk State University
Email: pyshnyi@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090; Novosibirsk, 630090
I. Pyshnaya
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: pyshnyi@niboch.nsc.ru
俄罗斯联邦, Novosibirsk, 630090