A Novel Method of Covalent Lysozyme Immobilization for the Development of Materials for Medical Applications
- Autores: Levashov P.1,2, Matolygina D.1,2, Ovchinnikova E.3, Adamova I.4, Dmitrieva O.3, Pokrovsky N.2,5, Eremeev N.1
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Afiliações:
- Faculty of Chemistry, Moscow State University
- Bauman State Technical University, Interdisciplinary Engineering Center for Composite Materials
- National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- POKARD Ltd
- Faculty of Fundamental Medicine, Moscow State University
- Edição: Volume 45, Nº 2 (2019)
- Páginas: 101-106
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229170
- DOI: https://doi.org/10.1134/S1068162019020055
- ID: 229170
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Resumo
Different methods of covalent lysozyme immobilization have been compared to choose an optimal approach to the development of a material suitable for medical applications in extracorporeal therapy. A novel method for lysozyme immobilization on a polymeric agarose matrix has been proposed, which provides the effective action of lysozyme on bacterial cells and eliminates the leakage of the enzyme from the material. The resultant immobilized lysozyme exhibits bacteriolytic activity toward Gram-positive bacteria Micrococcus luteus and Gram-negative bacteria Escherichia coli. During the immobilization of the enzyme, a broadening of the pH optimum of its activity occurs. The compatibility of immobilized lysozyme with human whole blood has been shown.
Sobre autores
P. Levashov
Faculty of Chemistry, Moscow State University; Bauman State Technical University, Interdisciplinary Engineering Center for Composite Materials
Autor responsável pela correspondência
Email: levashov@yahoo.com
Rússia, Moscow, 119234; Moscow, 105005
D. Matolygina
Faculty of Chemistry, Moscow State University; Bauman State Technical University, Interdisciplinary Engineering Center for Composite Materials
Email: levashov@yahoo.com
Rússia, Moscow, 119234; Moscow, 105005
E. Ovchinnikova
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: levashov@yahoo.com
Rússia, Moscow, 121359
I. Adamova
POKARD Ltd
Email: levashov@yahoo.com
Rússia, Moscow, 121359
O. Dmitrieva
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: levashov@yahoo.com
Rússia, Moscow, 121359
N. Pokrovsky
Bauman State Technical University, Interdisciplinary Engineering Center for Composite Materials; Faculty of Fundamental Medicine, Moscow State University
Email: levashov@yahoo.com
Rússia, Moscow, 105005; Moscow, 119991
N. Eremeev
Faculty of Chemistry, Moscow State University
Email: levashov@yahoo.com
Rússia, Moscow, 119234