Green fluorescent protein with tryptophan-based chromophore stable at low pH
- Autores: Gorbachev D.1,2, Sarkisyan K.1, Mishin A.1, Lukyanov K.1
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Afiliações:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Biology
- Edição: Volume 43, Nº 2 (2017)
- Páginas: 220-222
- Seção: Letter to the Editor
- URL: https://journals.rcsi.science/1068-1620/article/view/228498
- DOI: https://doi.org/10.1134/S1068162017010034
- ID: 228498
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Resumo
Using directed (substitution T203Y) and subsequent random mutagenesis of the monomeric cyan fluorescent protein mTurquoise2, we obtained a protein with a tryptophan-based chromophore that fluoresces in the green region of the spectrum (excitation maximum 482 nm, emission maximum 519 nm). Fluorescence of the new protein is highly stable in a wide range of pH (pKa 4.9), more stable than all monomeric green fluorescent proteins with a tyrosine-based chromophore.
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Sobre autores
D. Gorbachev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology
Email: kluk@ibch.ru
Rússia, Moscow, 117997; Moscow, 119991
K. Sarkisyan
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kluk@ibch.ru
Rússia, Moscow, 117997
A. Mishin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kluk@ibch.ru
Rússia, Moscow, 117997
K. Lukyanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Autor responsável pela correspondência
Email: kluk@ibch.ru
Rússia, Moscow, 117997