Efficiency of Bioluminescence Resonance Energy Transfer in the NanoLuc-miniSOG-Furimazine System
- Авторлар: Shramova E.1, Deyev S.1, Proshkina G.1
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Мекемелер:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Шығарылым: Том 44, № 6 (2018)
- Беттер: 755-758
- Бөлім: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229062
- DOI: https://doi.org/10.1134/S1068162018060080
- ID: 229062
Дәйексөз келтіру
Аннотация
Photodynamic therapy (PDT) is a clinically approved, minimally invasive method for tumor destruction in the presence of a photosensitizer (PS), oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is the problem of the excitation light delivery inside the body without reduction of light intensity. An internal light source based on a bioluminescence system NanoLuc-furimazine can be considered as an alternative to an external light source. This system can be successfully used to excite a protein photosensitizer miniSOG and induce its phototoxicity in cancer cells as a result of bioluminescent resonance energy transfer. In this paper, the effectiveness of bioluminescent resonance energy transfer in the NanoLuc-miniSOG-furimazine system in living cells was studied. The efficiency of the energy transfer in this system was found to be independent of the furimazine concentration, while intracellular localization of the NanoLuc-miniSOG hybrid protein was found to be a crucial factor.
Негізгі сөздер
Авторлар туралы
E. Shramova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Хат алмасуға жауапты Автор.
Email: shramova.e.i@gmail.com
Ресей, Moscow, 117997
S. Deyev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: shramova.e.i@gmail.com
Ресей, Moscow, 117997
G. Proshkina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: shramova.e.i@gmail.com
Ресей, Moscow, 117997