Mass Spectrometric Amino Acid Sequencing of Short and Mid-Sized Peptides in a ESI-O-TOF System as an Alternative to MS/MS: I. Selective Fragmentation of Peptides with y-Ion Formation

Аннотация

The possibility of mass spectrometric sequencing of peptides without the need for the conventional MS/MS analysis has been demonstrated experimentally. The peptide hydrolysate was fractionated by reversephase chromatography on a microbore column. The eluate fraction was injected into the mass spectrometer via an electrospray ion source that directly coupled a liquid chromatography instrument to a time-of-flight mass spectrometer (HPLC-MS). Fragmentation of the peptides eluted from the column was performed in the mass spectrometer interface by varying the voltage difference between the mass spectrometer nozzle and skimmer. A restricted set of intensive peaks of y-ions, which corresponded to sequential cleavage of all amino acids from the peptide, was obtained. The ratios of the y-ion peak intensities to the background were (5−100)/1. The presence of Lys and Arg in the peptides provided for a substantial increase of informative peak intensity in the mass spectra. The mass spectra of short peptides (up to 10 residues) were processed manually, whereas the Proteos hardware and software system was used to process the fragmentation results for a long N-terminal peptide of the human hemoglobin α-chain.