Electrochemical DNA sensors on the basis of electropolymerized thionine and Azure B with addition of pillar[5]arene as an electron transfer mediator
- Авторлар: Stoikov D.1, Porfir’eva A.1, Shurpik D.1, Stoikov I.1, Evtyugin G.1
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Мекемелер:
- Alexander Butlerov Institute of Chemistry, Kazan Federal University
- Шығарылым: Том 68, № 2 (2019)
- Беттер: 431-437
- Бөлім: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/243265
- DOI: https://doi.org/10.1007/s11172-019-2404-8
- ID: 243265
Дәйексөз келтіру
Аннотация
A DNA sensor was developed on the basis of glassy carbon electrode coated with polymeric forms of thionine and Azure B. Introduction of carbon black and pillar[5]arene into the electrode composition increases the efficiency of polymerization and the oxidation peak currents of dyes due to the mediating effect of the macrocycle. The addition of DNA onto the sensor surface and into the reaction mixture differently influences the electrochemical activity of poly(Azure B) and polythionine. The control of changes in current-voltage characteristics allowed us to identify the heat denaturation of DNA and its oxidation by reactive oxygen species generated upon the reaction of hydrogen peroxide and copper(II) salt. The DNA sensors can find application in the diagnosis of DNA damage on exposure to carcinogens and in screening of cytotoxic anticancer drugs.
Авторлар туралы
D. Stoikov
Alexander Butlerov Institute of Chemistry, Kazan Federal University
Email: Ivan.Stoikov@mail.ru
Ресей, 18 ul. Kremvlevskaya, Kazan, 420008
A. Porfir’eva
Alexander Butlerov Institute of Chemistry, Kazan Federal University
Хат алмасуға жауапты Автор.
Email: porfireva-a@inbox.ru
Ресей, 18 ul. Kremvlevskaya, Kazan, 420008
D. Shurpik
Alexander Butlerov Institute of Chemistry, Kazan Federal University
Email: Ivan.Stoikov@mail.ru
Ресей, 18 ul. Kremvlevskaya, Kazan, 420008
I. Stoikov
Alexander Butlerov Institute of Chemistry, Kazan Federal University
Хат алмасуға жауапты Автор.
Email: Ivan.Stoikov@mail.ru
Ресей, 18 ul. Kremvlevskaya, Kazan, 420008
G. Evtyugin
Alexander Butlerov Institute of Chemistry, Kazan Federal University
Email: Ivan.Stoikov@mail.ru
Ресей, 18 ul. Kremvlevskaya, Kazan, 420008