Enantioselective adsorption of dipeptides on chiral stationary phases with grafted macrocyclic antibiotics using glycylaspartic acid as an example

Abstract

The chromatographic behavior and adsorption of enantiomers of glycylaspartic acid on the Nautilus-E chiral stationary phase with grafted eremomycin antibiotic were studied. Acetate buf er mixtures with variable pH prepared in a water—methanol mixed solvent (volume ratio 60 : 40) were used as acetate buf ers. The retention of Gly—Asp was found to be enantioselective and depending on the ratio of various ionic forms of dipeptide in the mobile phase. The ratio is determined by the pH. A model of adsorption equilibrium assuming independent binding of diverse ionic forms with the stationary phase, which is characteristic of energy uniformity, was proposed. This model is well consistent with the experimental data. Features of application of the equilibrium dispersion and transport dispersion dynamic elution models for the determination of the adsorption isotherms by the inverse method are discussed.