Specific properties of hydroxyapatite as a potential transporter of copper ions and its complexes

Abstract

Modification of the cocrystallization method for producing hydroxyapatite (HAP) and an HAP-Cu combination to the enzymatic method using alkaline phosphatase leads to a change in the morphology, sizes, sorption capacity, type of particles, and conformity with the Langmuir and Freundlich models. A positive factor of the enzyme usage is an increase in the sorption capacity and the possibility to strictly control the particle sizes depending on the concentration of the enzyme used. The L₂CuCl₄ complex was synthesized on the basis of 2-aminopyrimidine (L), which is the precursor of many anticancer drugs, and the possibilities of introducing L₂CuCl₄ into the HAP composite were considered. The cytotoxicity data for various HAP and L₂CuCl₄ composites with respect to various types of leukemic cells as compared to lymphocytes of healthy donors showed antileukemic activity of the copper complex and the absence of HAP cytotoxicity in a wide range of concentrations.