Immunoradiometric Assay for In Vitro Determination of Prostate Specific Antigen (PSA) in Human Serum Using Solid Phase Anti-PSA Coated Tubes
- Autores: El-Bayoumy A.1, Sallam K.1, Mehany N.1
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Afiliações:
- Department of Labeled Compounds, Hot Laboratories Center
- Edição: Volume 60, Nº 4 (2018)
- Páginas: 427-433
- Seção: Article
- URL: https://journals.rcsi.science/1066-3622/article/view/224633
- DOI: https://doi.org/10.1134/S1066362218040124
- ID: 224633
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Resumo
An immunoradiometric assay (IRMA) procedure for estimation of prostate specific antigen (PSA) using coated tubes was developed, optimized, and validated. 125I-PSA and 125I-anti-hPSA tracers were prepared using iodogen and lactoperoxidase methods, respectively. Production of polyclonal anti-PSA was carried out using four New Zealand rabbits as a host. The polyclonal antibody was purified using caprylic acid/ammonium sulfate precipitation method. The coating was performed using the purified polyclonal antibody. The assay is highly sensitive and accurate.
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Sobre autores
A. El-Bayoumy
Department of Labeled Compounds, Hot Laboratories Center
Autor responsável pela correspondência
Email: asami1972@mail.ru
Egito, Cairo
Kh. Sallam
Department of Labeled Compounds, Hot Laboratories Center
Email: asami1972@mail.ru
Egito, Cairo
N. Mehany
Department of Labeled Compounds, Hot Laboratories Center
Email: asami1972@mail.ru
Egito, Cairo