Immunoradiometric Assay for In Vitro Determination of Prostate Specific Antigen (PSA) in Human Serum Using Solid Phase Anti-PSA Coated Tubes
- Authors: El-Bayoumy A.S.1, Sallam K.M.1, Mehany N.L.1
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Affiliations:
- Department of Labeled Compounds, Hot Laboratories Center
- Issue: Vol 60, No 4 (2018)
- Pages: 427-433
- Section: Article
- URL: https://journals.rcsi.science/1066-3622/article/view/224633
- DOI: https://doi.org/10.1134/S1066362218040124
- ID: 224633
Cite item
Abstract
An immunoradiometric assay (IRMA) procedure for estimation of prostate specific antigen (PSA) using coated tubes was developed, optimized, and validated. 125I-PSA and 125I-anti-hPSA tracers were prepared using iodogen and lactoperoxidase methods, respectively. Production of polyclonal anti-PSA was carried out using four New Zealand rabbits as a host. The polyclonal antibody was purified using caprylic acid/ammonium sulfate precipitation method. The coating was performed using the purified polyclonal antibody. The assay is highly sensitive and accurate.
About the authors
A. S. A. El-Bayoumy
Department of Labeled Compounds, Hot Laboratories Center
Author for correspondence.
Email: asami1972@mail.ru
Egypt, Cairo
Kh. M. Sallam
Department of Labeled Compounds, Hot Laboratories Center
Email: asami1972@mail.ru
Egypt, Cairo
N. L. Mehany
Department of Labeled Compounds, Hot Laboratories Center
Email: asami1972@mail.ru
Egypt, Cairo