Three-dimensional structure of E. Coli purine nucleoside phosphorylase at 0.99 Å resolution
- Authors: Timofeev V.I.1,2, Abramchik Y.A.3, Zhukhlistova N.E.1, Muravieva T.I.3, Esipov R.S.3, Kuranova I.P.1
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Affiliations:
- Shubnikov Institute of Crystallography
- National Research Centre “Kurchatov Institute,”
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 61, No 2 (2016)
- Pages: 249-257
- Section: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/189880
- DOI: https://doi.org/10.1134/S1063774516020292
- ID: 189880
Cite item
Abstract
Purine nucleoside phosphorylases (PNPs) catalyze the reversible phosphorolysis of nucleosides and are key enzymes involved in nucleotide metabolism. They are essential for normal cell function and can catalyze the transglycosylation. Crystals of E. coli PNP were grown in microgravity by the capillary counterdiffusion method through a gel layer. The three-dimensional structure of the enzyme was determined by the molecular-replacement method at 0.99 Å resolution. The structural features are considered, and the structure of E. coli PNP is compared with the structures of the free enzyme and its complexes with purine base derivatives established earlier. A comparison of the environment of the purine base in the complex of PNP with formycin A and of the pyrimidine base in the complex of uridine phosphorylase with thymidine revealed the main structural features of the base-binding sites. Coordinates of the atomic model determined with high accuracy were deposited in the Protein Data Bank (PDB_ID: 4RJ2).
About the authors
V. I. Timofeev
Shubnikov Institute of Crystallography; National Research Centre “Kurchatov Institute,”
Author for correspondence.
Email: tostars@mail.ru
Russian Federation, Leninskii pr. 59, Moscow, 119333; pl. Akademika Kurchatova 1, Moscow, 123098
Yu. A. Abramchik
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tostars@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
N. E. Zhukhlistova
Shubnikov Institute of Crystallography
Email: tostars@mail.ru
Russian Federation, Leninskii pr. 59, Moscow, 119333
T. I. Muravieva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tostars@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
R. S. Esipov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tostars@mail.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
I. P. Kuranova
Shubnikov Institute of Crystallography
Email: tostars@mail.ru
Russian Federation, Leninskii pr. 59, Moscow, 119333