Possibilities of X-Ray Absorption Spectroscopy in the Total External Reflection Geometry for Studying Protein Films on Liquids
- Authors: Novikova N.N.1, Yakunin S.N.1, Koval’chuk M.V.1, Yur’eva E.A.2, Stepina N.D.3, Rogachev A.V.1, Kremennaya M.A.4, Yalovega G.E.4, Kosmachevskaya O.V.5, Topunov A.F.5
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Affiliations:
- National Research Centre “Kurchatov Institute”
- Veltischev Research and Clinical Institute for Pediatrics
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- Southern Federal University
- Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences
- Issue: Vol 64, No 6 (2019)
- Pages: 952-957
- Section: Surface and Thin Films
- URL: https://journals.rcsi.science/1063-7745/article/view/194342
- DOI: https://doi.org/10.1134/S1063774519060130
- ID: 194342
Cite item
Abstract
XANES spectra of protein films (hemoglobin and alkaline phosphatase) formed on the surface of a liquid subphase in a Langmuir trough have been obtained experimentally. The potential of X-ray absorption spectroscopy in the total external reflection geometry is demonstrated by the example of a protein film based on hemoglobin subjected to the action of urea. It is established that the presence of 0.09 M urea solution in the subphase enhances significantly the ability of hemoglobin to bind zinc and iron ions. Information about the local atomic environment of zinc ions bound with a hemoglobin molecule is obtained. It is shown that each zinc ion is coordinated by four ligands, two of which are amino acid residues of cysteine and histidine. The general concepts of the molecular mechanisms of accumulation of metal ions under the action of disturbing factors are formulated.
About the authors
N. N. Novikova
National Research Centre “Kurchatov Institute”
Author for correspondence.
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 123182
S. N. Yakunin
National Research Centre “Kurchatov Institute”
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 123182
M. V. Koval’chuk
National Research Centre “Kurchatov Institute”
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 123182
E. A. Yur’eva
Veltischev Research and Clinical Institute for Pediatrics
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 117997
N. D. Stepina
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 119333
A. V. Rogachev
National Research Centre “Kurchatov Institute”
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 123182
M. A. Kremennaya
Southern Federal University
Email: nn-novikova07@yandex.ru
Russian Federation, Rostov-on-Don, 344006
G. E. Yalovega
Southern Federal University
Email: nn-novikova07@yandex.ru
Russian Federation, Rostov-on-Don, 344006
O. V. Kosmachevskaya
Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,”Russian Academy of Sciences
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 119071
A. F. Topunov
Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,”Russian Academy of Sciences
Email: nn-novikova07@yandex.ru
Russian Federation, Moscow, 119071