Enviar artigo por via de e-mail Comparative Analysis of Different Methods of Scanning Electron Microscopy and Test Preparation in Biological Tissue Studies
- Autores: Kamyshinskii R.A.1,2,3, Orekhov A.S.1,2, Grigor’ev T.E.1, Kuevda E.V.4, Gubareva E.A.4, Tokaev K.V.5, Chvalun S.N.1, Vasil’ev A.L.1,2,3
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Afiliações:
- National Research Centre “Kurchatov Institute”
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences
- Moscow Institute of Physics and Technology (State University)
- Kuban State Medical University, Ministry of Health of the Russian Federation
- National Research Medical Center of Phthisiopulmonology and Infection Diseases, Ministry of Health of the Russian Federation
- Edição: Volume 64, Nº 3 (2019)
- Páginas: 466-469
- Seção: Nanomaterials and Ceramics
- URL: https://journals.rcsi.science/1063-7745/article/view/193989
- DOI: https://doi.org/10.1134/S106377451903012X
- ID: 193989
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Resumo
Different methods of scanning electron microscopy in combination with different sample preparation techniques have been compared by an example of decellularized rat diaphragm matrices. It is shown that a combination of scanning electron microscopy under natural environmental conditions, allowing one to investigate tissue samples in a state similar to native, with the conventional high-vacuum microscopy of samples obtained by drying at the critical point, provides the most complete microstructural analysis of native and decellularized tissue samples.
Sobre autores
R. Kamyshinskii
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)
Email: a.vasiliev56@gmail.com
Rússia, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701
A. Orekhov
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences
Email: a.vasiliev56@gmail.com
Rússia, Moscow, 123182; Moscow, 119333
T. Grigor’ev
National Research Centre “Kurchatov Institute”
Email: a.vasiliev56@gmail.com
Rússia, Moscow, 123182
E. Kuevda
Kuban State Medical University, Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Rússia, Krasnodar
E. Gubareva
Kuban State Medical University, Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Rússia, Krasnodar
K. Tokaev
National Research Medical Center of Phthisiopulmonology and Infection Diseases,Ministry of Health of the Russian Federation
Email: a.vasiliev56@gmail.com
Rússia, Moscow
S. Chvalun
National Research Centre “Kurchatov Institute”
Email: a.vasiliev56@gmail.com
Rússia, Moscow, 123182
A. Vasil’ev
National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)
Autor responsável pela correspondência
Email: a.vasiliev56@gmail.com
Rússia, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701
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