Comparative Analysis of Different Methods of Scanning Electron Microscopy and Test Preparation in Biological Tissue Studies


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Different methods of scanning electron microscopy in combination with different sample preparation techniques have been compared by an example of decellularized rat diaphragm matrices. It is shown that a combination of scanning electron microscopy under natural environmental conditions, allowing one to investigate tissue samples in a state similar to native, with the conventional high-vacuum microscopy of samples obtained by drying at the critical point, provides the most complete microstructural analysis of native and decellularized tissue samples.

作者简介

R. Kamyshinskii

National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701

A. Orekhov

National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow, 123182; Moscow, 119333

T. Grigor’ev

National Research Centre “Kurchatov Institute”

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow, 123182

E. Kuevda

Kuban State Medical University, Ministry of Health of the Russian Federation

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Krasnodar

E. Gubareva

Kuban State Medical University, Ministry of Health of the Russian Federation

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Krasnodar

K. Tokaev

National Research Medical Center of Phthisiopulmonology and Infection Diseases,
Ministry of Health of the Russian Federation

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow

S. Chvalun

National Research Centre “Kurchatov Institute”

Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow, 123182

A. Vasil’ev

National Research Centre “Kurchatov Institute”; Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,”
Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University)

编辑信件的主要联系方式.
Email: a.vasiliev56@gmail.com
俄罗斯联邦, Moscow, 123182; Moscow, 119333; Dolgoprudny, Moscow oblast, 141701

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