Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster
- 作者: Boyko K.M.1,2, Nikolaeva A.Y.1,2, Kachalova G.S.2, Bonchuk A.N.3, Dorovatovskii P.V.2, Popov V.O.1,2
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隶属关系:
- Federal Research Centre “Fundamentals of Biotechnology,”
- National Research Centre “Kurchatov Institute,”
- Institute of Gene Biology
- 期: 卷 62, 编号 6 (2017)
- 页面: 912-915
- 栏目: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/191493
- DOI: https://doi.org/10.1134/S1063774517060062
- ID: 191493
如何引用文章
详细
The Drosophila genome has several dozens of transcription factors (TTK group) containing ВТВ domains assembled into octamers. The LOLA protein belongs to this family. The purification, crystallization, and preliminary X-ray diffraction and small-angle X-ray scattering (SAXS) studies of the BTB domain of this protein are reported. The crystallization conditions were found by the vapor-diffusion technique. A very low diffraction resolution (8.7 Å resolution) of the crystals was insufficient for the determination of the threedimensional structure of the BTB domain. The SAXS study demonstrated that the BTB domain of the LOLA protein exists as an octamer in solution.
作者简介
K. Boyko
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
编辑信件的主要联系方式.
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 119071; Moscow, 123098
A. Nikolaeva
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 119071; Moscow, 123098
G. Kachalova
National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 123098
A. Bonchuk
Institute of Gene Biology
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 119334
P. Dorovatovskii
National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 123098
V. Popov
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
俄罗斯联邦, Moscow, 119071; Moscow, 123098
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