Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster
- Авторы: Boyko K.M.1,2, Nikolaeva A.Y.1,2, Kachalova G.S.2, Bonchuk A.N.3, Dorovatovskii P.V.2, Popov V.O.1,2
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Учреждения:
- Federal Research Centre “Fundamentals of Biotechnology,”
- National Research Centre “Kurchatov Institute,”
- Institute of Gene Biology
- Выпуск: Том 62, № 6 (2017)
- Страницы: 912-915
- Раздел: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/191493
- DOI: https://doi.org/10.1134/S1063774517060062
- ID: 191493
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Аннотация
The Drosophila genome has several dozens of transcription factors (TTK group) containing ВТВ domains assembled into octamers. The LOLA protein belongs to this family. The purification, crystallization, and preliminary X-ray diffraction and small-angle X-ray scattering (SAXS) studies of the BTB domain of this protein are reported. The crystallization conditions were found by the vapor-diffusion technique. A very low diffraction resolution (8.7 Å resolution) of the crystals was insufficient for the determination of the threedimensional structure of the BTB domain. The SAXS study demonstrated that the BTB domain of the LOLA protein exists as an octamer in solution.
Об авторах
K. Boyko
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
Автор, ответственный за переписку.
Email: kmb@inbi.ras.ru
Россия, Moscow, 119071; Moscow, 123098
A. Nikolaeva
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
Россия, Moscow, 119071; Moscow, 123098
G. Kachalova
National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
Россия, Moscow, 123098
A. Bonchuk
Institute of Gene Biology
Email: kmb@inbi.ras.ru
Россия, Moscow, 119334
P. Dorovatovskii
National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
Россия, Moscow, 123098
V. Popov
Federal Research Centre “Fundamentals of Biotechnology,”; National Research Centre “Kurchatov Institute,”
Email: kmb@inbi.ras.ru
Россия, Moscow, 119071; Moscow, 123098
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