Structural investigation of endoglucanase 2 from the filamentous fungus Penicillium verruculosum
- Authors: Vakhrusheva A.V.1,2, Nemashkalov V.A.3, Kravchenko O.V.1, Tishchenko S.V.1, Gabdulkhakov A.G.1, Kljashtorny V.G.1, Korotkova O.G.4, Gusakov A.V.4,2, Sinitsyn A.P.4,2
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Affiliations:
- Institute of Protein Research
- Department of Chemistry
- Institute of Biochemistry and Physiology of Microorganisms
- Federal Research Centre “Fundamentals of Biotechnology”
- Issue: Vol 62, No 2 (2017)
- Pages: 254-259
- Section: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/190801
- DOI: https://doi.org/10.1134/S1063774517020304
- ID: 190801
Cite item
Abstract
Enzyme additives capable of degrading non-starch polysaccharides of cereal cell walls, which are major ingredients used in animal feed, can improve the efficiency of livestock production. Non-starch polysaccharides have antinutritional properties that interfere with efficient digestion and assimilation of nutrients by animals. Therefore, the improvement of the properties and characteristics of enzyme additive is an important issue. The three-dimensional structure of one of the key industrial enzymes involved in the degradation of non-starch polysaccharides — endoglucanase 2 from the filamentous fungus Penicillium verruculosum — was determined (PDB ID: 5I6S). The catalytic site of this enzyme was established. Based on the enzyme structure, it was suggested that the pH optimum of the enzyme activity can be shifted from acidic to neutral or alkaline pH values.
About the authors
A. V. Vakhrusheva
Institute of Protein Research; Department of Chemistry
Author for correspondence.
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 119991
V. A. Nemashkalov
Institute of Biochemistry and Physiology of Microorganisms
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290
O. V. Kravchenko
Institute of Protein Research
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290
S. V. Tishchenko
Institute of Protein Research
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290
A. G. Gabdulkhakov
Institute of Protein Research
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290
V. G. Kljashtorny
Institute of Protein Research
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290
O. G. Korotkova
Federal Research Centre “Fundamentals of Biotechnology”
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Moscow, 119071
A. V. Gusakov
Federal Research Centre “Fundamentals of Biotechnology”; Department of Chemistry
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Moscow, 119071; Moscow, 119991
A. P. Sinitsyn
Federal Research Centre “Fundamentals of Biotechnology”; Department of Chemistry
Email: vakhrusheva.ann@yandex.ru
Russian Federation, Moscow, 119071; Moscow, 119991