Structural investigation of endoglucanase 2 from the filamentous fungus Penicillium verruculosum


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Resumo

Enzyme additives capable of degrading non-starch polysaccharides of cereal cell walls, which are major ingredients used in animal feed, can improve the efficiency of livestock production. Non-starch polysaccharides have antinutritional properties that interfere with efficient digestion and assimilation of nutrients by animals. Therefore, the improvement of the properties and characteristics of enzyme additive is an important issue. The three-dimensional structure of one of the key industrial enzymes involved in the degradation of non-starch polysaccharides — endoglucanase 2 from the filamentous fungus Penicillium verruculosum — was determined (PDB ID: 5I6S). The catalytic site of this enzyme was established. Based on the enzyme structure, it was suggested that the pH optimum of the enzyme activity can be shifted from acidic to neutral or alkaline pH values.

Sobre autores

A. Vakhrusheva

Institute of Protein Research; Department of Chemistry

Autor responsável pela correspondência
Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290; Moscow, 119991

V. Nemashkalov

Institute of Biochemistry and Physiology of Microorganisms

Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290

O. Kravchenko

Institute of Protein Research

Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290

S. Tishchenko

Institute of Protein Research

Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290

A. Gabdulkhakov

Institute of Protein Research

Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290

V. Kljashtorny

Institute of Protein Research

Email: vakhrusheva.ann@yandex.ru
Rússia, Pushchino, Moscow oblast, 142290

O. Korotkova

Federal Research Centre “Fundamentals of Biotechnology”

Email: vakhrusheva.ann@yandex.ru
Rússia, Moscow, 119071

A. Gusakov

Federal Research Centre “Fundamentals of Biotechnology”; Department of Chemistry

Email: vakhrusheva.ann@yandex.ru
Rússia, Moscow, 119071; Moscow, 119991

A. Sinitsyn

Federal Research Centre “Fundamentals of Biotechnology”; Department of Chemistry

Email: vakhrusheva.ann@yandex.ru
Rússia, Moscow, 119071; Moscow, 119991

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