Hemolymph cholinesterase activity in the Mussel Crenomytilus grayanus (Dunker, 1853) that was exposed to adverse natural and anthropogenic conditions
- Authors: Kovalev N.N.1,2, Kavun V.Y.3, Kostetsky E.Y.1, Mikheev Y.V.2, Podgurskaya O.V.1,3
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Affiliations:
- Far Eastern Federal University
- Far Eastern State Technical Fisheries University
- Zhirmunsky Institute of Marine Biology, Far East Branch
- Issue: Vol 42, No 1 (2016)
- Pages: 65-72
- Section: Biochemical Adaptation
- URL: https://journals.rcsi.science/1063-0740/article/view/183532
- DOI: https://doi.org/10.1134/S1063074016010120
- ID: 183532
Cite item
Abstract
The influence of habitat conditions on the activity, the structure of the substrate specificity (the ratio of the substrate hydrolysis rates), and the kinetic parameters of substrate hydrolysis due to the effect of hemolymph cholinesterase of the mussel Crenomytilus grayanus was studied. Mussels were collected from areas that are influenced by seasonal and stationary upwelling, as well as from a polluted area. Upwelling and anthropogenic pressure were shown to alter the structure of hemolymph cholinesterase substrate specificity in mussels, up to complete loss of the ability to catalyze the hydrolysis of propionyland butyrylthiocholine. It was established that during the seasonal upwelling the efficiency of the cholinergic process in mussels is provided by a wide range of effective concentrations of the substrates and by decreasing their affinity to the enzyme. Under the conditions of chronic anthropogenic pollution, the cholinesterase of the mussel hemolymph loses its ability to hydrolyze substrates other than acetylthiocholine.
About the authors
N. N. Kovalev
Far Eastern Federal University; Far Eastern State Technical Fisheries University
Email: vkavun11@mail.ru
Russian Federation, ul. Sukhanova 8, Vladivostok, 690950; ul. Lugovaya 52b, Vladivostok, 690087
V. Ya. Kavun
Zhirmunsky Institute of Marine Biology, Far East Branch
Author for correspondence.
Email: vkavun11@mail.ru
Russian Federation, ul. Pal’chevskogo 17, Vladivostok, 690041
E. Ya. Kostetsky
Far Eastern Federal University
Email: vkavun11@mail.ru
Russian Federation, ul. Sukhanova 8, Vladivostok, 690950
Ye. V. Mikheev
Far Eastern State Technical Fisheries University
Email: vkavun11@mail.ru
Russian Federation, ul. Lugovaya 52b, Vladivostok, 690087
O. V. Podgurskaya
Far Eastern Federal University; Zhirmunsky Institute of Marine Biology, Far East Branch
Email: vkavun11@mail.ru
Russian Federation, ul. Sukhanova 8, Vladivostok, 690950; ul. Pal’chevskogo 17, Vladivostok, 690041