Preservation of a specialized phenotype of dermal papilla cells of a human hair follicle under cultivation conditions
- Авторы: Kalabusheva E.1,2, Chermnykh E.1,2, Terskikh V.1, Vorotelyak E.1,2,3
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Учреждения:
- Koltzov Institute of Developmental Biology
- Pirogov Russian National Research Medical University
- Faculty of Biology
- Выпуск: Том 44, № 4 (2017)
- Страницы: 363-371
- Раздел: Cell Biology
- URL: https://journals.rcsi.science/1062-3590/article/view/182423
- DOI: https://doi.org/10.1134/S1062359017040069
- ID: 182423
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Аннотация
Possible ways to extend cultivation of dermal papilla cells without the loss of expression of their specific markers were studied. The effect of extracellular matrix components, as well as valproic acid, on the maintenance of the phenotype of dermal papilla cells was studied for the first time. Two ways of cultivation (in a monolayer culture and in spheroids) were used. It was established that a short-term positive effect is reached during the addition of the BMP6 growth factor and vitamin D3 in the monolayer culture, while cultivation in spheroids or in the presence of valproic acid allows us to preserve most efficiently the initial phenotype of these cells in vitro. The significance of the results obtained for tissue engineering and for the study of regeneration processes is discussed.
Об авторах
E. Kalabusheva
Koltzov Institute of Developmental Biology; Pirogov Russian National Research Medical University
Автор, ответственный за переписку.
Email: kalabusheva.e@gmail.com
Россия, ul. Vavilova 26, Moscow, 119334; ul. Ostrovityanova 1, Moscow, 117997
E. Chermnykh
Koltzov Institute of Developmental Biology; Pirogov Russian National Research Medical University
Email: kalabusheva.e@gmail.com
Россия, ul. Vavilova 26, Moscow, 119334; ul. Ostrovityanova 1, Moscow, 117997
V. Terskikh
Koltzov Institute of Developmental Biology
Email: kalabusheva.e@gmail.com
Россия, ul. Vavilova 26, Moscow, 119334
E. Vorotelyak
Koltzov Institute of Developmental Biology; Pirogov Russian National Research Medical University; Faculty of Biology
Email: kalabusheva.e@gmail.com
Россия, ul. Vavilova 26, Moscow, 119334; ul. Ostrovityanova 1, Moscow, 117997; Moscow, 119234