Cytogenetic Activity of Gold Nanoparticles in Germ and Somatic Cells of 129 Mice with a Nonsense Mutation in the DNA Polymerase Iota Gene
- Authors: Mudzhiri N.M.1,2, Zakhidov S.T.1, Rudoy V.M.3, Dement’eva O.V.3, Makarov A.A.4, Makarova I.V.5, Zelenina I.A.1, Andreeva L.E.5, Marshak T.L.2
-
Affiliations:
- Faculty of Biology
- Koltzov Institute of Developmental Biology
- Frumkin Institute of Physical Chemistry and Electrochemistry
- Higher School of Economics National Research University
- Institute of Molecular Genetics
- Issue: Vol 45, No 2 (2018)
- Pages: 119-125
- Section: Developmental Biology
- URL: https://journals.rcsi.science/1062-3590/article/view/182721
- DOI: https://doi.org/10.1134/S1062359018020085
- ID: 182721
Cite item
Abstract
In this study, we investigated the cytogenetic effects of single and quadruple exposure of spermatogenic cells and hepatocytes of 129 mice, which have a mutation in the gene that encodes DNA polymerase iota, to ultrasmall gold nanoparticles (GNPs). The combined effects of GNPs and chemical mutagen dipin were evaluated. In all cases, except for the experiment with the quadruple GNP injection, we observed a slight, statistically nonsignificant increase in the frequency of round spermatids with micronuclei compared to the negative control (saline). It is established that, in the intact liver of 129 mice, in all variants of the experiment, GNPs behaved as a potentially cytotoxic agent, as evidenced by the decrease in the frequency of the micronucleated hepatocytes.
About the authors
N. M. Mudzhiri
Faculty of Biology; Koltzov Institute of Developmental Biology
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119991; Moscow, 119334
S. T. Zakhidov
Faculty of Biology
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119991
V. M. Rudoy
Frumkin Institute of Physical Chemistry and Electrochemistry
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119071
O. V. Dement’eva
Frumkin Institute of Physical Chemistry and Electrochemistry
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119071
A. A. Makarov
Higher School of Economics National Research University
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 101000
I. V. Makarova
Institute of Molecular Genetics
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 123182
I. A. Zelenina
Faculty of Biology
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119991
L. E. Andreeva
Institute of Molecular Genetics
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 123182
T. L. Marshak
Koltzov Institute of Developmental Biology
Author for correspondence.
Email: 4361.idb@bk.ru
Russian Federation, Moscow, 119334