The role of protein oxidative modification and the cellular redox status in realization of apoptosis of MCF-7 breast adenocarcinoma cells
- Authors: Shakhristova E.V.1, Stepovaya E.A.1, Ryazantseva N.V.2, Nosareva O.L.1, Yakushina V.D.1, Ivanov V.V.1, Novitsky V.V.1
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Affiliations:
- Siberian State Medical University
- Siberian Federal University
- Issue: Vol 43, No 5 (2016)
- Pages: 385-389
- Section: Biochemistry
- URL: https://journals.rcsi.science/1062-3590/article/view/181631
- DOI: https://doi.org/10.1134/S1062359016050095
- ID: 181631
Cite item
Abstract
The aim of this study was to establish the role of oxidative modification of proteins and redox status in the realization of apoptosis of MCF-7 breast adenocarcinoma cells during cultivation with the SH-group blocker N-ethylmaleimide (NEM) and the SH-group protector 1,4-dithioerythritol (DTE). The activation of apoptosis in MCF-7 breast adenocarcinoma cells was shown to be due to the irreversible modification of redox-sensitive protein molecules. The presence of DTE in the culture medium of cancer cells caused reversible glutathionylation of protein molecules and did not change the number of apoptotic MCF-7 cells.
About the authors
E. V. Shakhristova
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050
E. A. Stepovaya
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050
N. V. Ryazantseva
Siberian Federal University
Author for correspondence.
Email: shaxristova@yandex.ru
Russian Federation, Svobodnyi pr. 79, Krasnoyarsk, 660041
O. L. Nosareva
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050
V. D. Yakushina
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050
V. V. Ivanov
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050
V. V. Novitsky
Siberian State Medical University
Email: shaxristova@yandex.ru
Russian Federation, Moskovskii trakt 2, Tomsk, 634050