The role of protein oxidative modification and the cellular redox status in realization of apoptosis of MCF-7 breast adenocarcinoma cells
- Autores: Shakhristova E.1, Stepovaya E.1, Ryazantseva N.2, Nosareva O.1, Yakushina V.1, Ivanov V.1, Novitsky V.1
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Afiliações:
- Siberian State Medical University
- Siberian Federal University
- Edição: Volume 43, Nº 5 (2016)
- Páginas: 385-389
- Seção: Biochemistry
- URL: https://journals.rcsi.science/1062-3590/article/view/181631
- DOI: https://doi.org/10.1134/S1062359016050095
- ID: 181631
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Resumo
The aim of this study was to establish the role of oxidative modification of proteins and redox status in the realization of apoptosis of MCF-7 breast adenocarcinoma cells during cultivation with the SH-group blocker N-ethylmaleimide (NEM) and the SH-group protector 1,4-dithioerythritol (DTE). The activation of apoptosis in MCF-7 breast adenocarcinoma cells was shown to be due to the irreversible modification of redox-sensitive protein molecules. The presence of DTE in the culture medium of cancer cells caused reversible glutathionylation of protein molecules and did not change the number of apoptotic MCF-7 cells.
Sobre autores
E. Shakhristova
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050
E. Stepovaya
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050
N. Ryazantseva
Siberian Federal University
Autor responsável pela correspondência
Email: shaxristova@yandex.ru
Rússia, Svobodnyi pr. 79, Krasnoyarsk, 660041
O. Nosareva
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050
V. Yakushina
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050
V. Ivanov
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050
V. Novitsky
Siberian State Medical University
Email: shaxristova@yandex.ru
Rússia, Moskovskii trakt 2, Tomsk, 634050