Dispersive Liquid–Liquid Microextraction for the Determination of Salivary Melatonin as a Biomarker of Circadian Rhythm
- 作者: Pourhossein M.1, Shahtaheri S.2,3, Mazloumi A.1, Rahimi-Foroushani A.4, Helmi-Kohneshahri M.1, Khani H.5
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隶属关系:
- Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences
- Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences
- Institute for Environmental Research, Tehran University of Medical Sciences
- Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences
- Department of Occupational Health Engineering, School of Public Health, Mazandaran University of Medical Sciences
- 期: 卷 73, 编号 10 (2018)
- 页面: 966-972
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182844
- DOI: https://doi.org/10.1134/S106193481810009X
- ID: 182844
如何引用文章
详细
Melatonin (N-acetyl-methoxytryptamine) is secreted from a part of the brain called suprachiasmatic nuclei. The biological clock of the body or the circadian rhythm is regulated through the secretion of hormones such as melatonin and cortisol. The dispersive liquid–liquid microextraction (DLLME) was optimized for the determination of human salivary melatonin. Different variables including the nature of extracting and dispersing solvent, their volumes, sample pH, ionic strength, extraction and centrifugation times were screened using One Factor At a Time design and then, the significant variables were selected as optimum values. Accuracy and precision of the optimized method were evaluated at concentrations of 50, 100, 250 pg/mL by achieving CV% for day-to-day and within-day reproducibility. Considering the appropriate results of the study, DLLME procedure can be used for the determination of melatonin hormone in saliva samples.
作者简介
M. Pourhossein
Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Tehran, 141556445
S. Shahtaheri
Department of Occupational Health Engineering, School of Public Health,Tehran University of Medical Sciences; Institute for Environmental Research, Tehran University of Medical Sciences
编辑信件的主要联系方式.
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Tehran, 141556445;
Tehran, 141556445
A. Mazloumi
Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Tehran, 141556445
A. Rahimi-Foroushani
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Tehran, 141556445
M. Helmi-Kohneshahri
Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Tehran, 141556445
H. Khani
Department of Occupational Health Engineering, School of Public Health, Mazandaran University of Medical Sciences
Email: shahtaheri@tums.ac.ir
伊朗伊斯兰共和国, Sari, 549156851
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