Phospholipid Composition of Human Blood Plasma as Detected by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: New Observations
- 作者: Milman B.1, Lugovkina N.1,2, Zhurkovich I.2
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隶属关系:
- Institute of Experimental Medicine
- Institute of Toxicology
- 期: 卷 72, 编号 14 (2017)
- 页面: 1411-1418
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182753
- DOI: https://doi.org/10.1134/S1061934817140088
- ID: 182753
如何引用文章
详细
The dependence of matrix-assisted laser desorption/ionization (MALDI) mass spectra of lipids extracted from human blood plasma and corresponding lipid compositions on different experimental conditions was studied. Not only numerous compounds of the lysophosphatidylcholines (LPCs) and phosphatidylcholines (PCs) groups, but also lipid oxidation products, including six oxidized LPCs, are detected. They are present in the original samples and produced by the reaction with air oxygen molecules, resulted in their accumulation in samples under analysis. A higher sensitivity of MALDI detection is observed for less hydrophobic LPCs as compared to more hydrophobic PCs; this phenomenon is explained. The hydrophobicity/ lipophilicity factor is also revealed in replacing the matrix of 2,5-dihydroxybenzoic acid by α-cyano-4- hydroxycinnamic acid. The latter matrix is rarely used in MALDI detection of lipids and may be suitable for the selective determination of LPCs and oxidized LPCs.
作者简介
B. Milman
Institute of Experimental Medicine
编辑信件的主要联系方式.
Email: bmilman@mail.rcom.ru
俄罗斯联邦, St. Petersburg, 197376
N. Lugovkina
Institute of Experimental Medicine; Institute of Toxicology
Email: bmilman@mail.rcom.ru
俄罗斯联邦, St. Petersburg, 197376; St. Petersburg, 192019
I. Zhurkovich
Institute of Toxicology
Email: bmilman@mail.rcom.ru
俄罗斯联邦, St. Petersburg, 192019
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