A Fast, Sensitive and Validated Technique for Eurycomanone and Cordycepin Quantitation using UPLC‒MS/MS
- Авторы: Chua L.1,2, Latiff N.1, Ware I.1
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Учреждения:
- Metabolites Profiling Laboratory, Institute of Bioproduct Development
- Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering
- Выпуск: Том 73, № 2 (2018)
- Страницы: 167-175
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182971
- DOI: https://doi.org/10.1134/S1061934818020065
- ID: 182971
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Аннотация
Herbs and herbal based products have been widely used, but some products might contain no herbal ingredient as claimed in the product label. Therefore, it is crucial to develop a fast, sensitive and reliable method to analyze the herbs and their finished products. The roots of Eurycoma longifolia, and the health supplement of E. longifolia and Cordyceps were used as the sample matrices in this study. Sonication assisted extraction was applied to extract the marker compounds, namely eurycomanone and cordycepin from E. longifolia and Cordyceps, respectively. The presence of the marker compounds was established by high throughput ultra-performance liquid chromatography tandem mass spectrometry (UPLC‒MS/MS) using a highly sensitive and selective technique of multiple reaction monitoring (MRM). The positive ion transitions in MRM for eurycomanone and cordycepin are m/z 409 → 391 and m/z 252 → 136, respectively. The test method was validated for its robustness, accuracy, precision, linearity, detection and quantitation limits, as well as estimated for its measurement uncertainty.
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Об авторах
Lee Chua
Metabolites Profiling Laboratory, Institute of Bioproduct Development; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering
Автор, ответственный за переписку.
Email: chualeesuan@utm.my
Малайзия, UTM Skudai, Johor Bahru, Johor, 81310; UTM Skudai, Johor Bahru, Johor, 81310
Norliza Latiff
Metabolites Profiling Laboratory, Institute of Bioproduct Development
Email: chualeesuan@utm.my
Малайзия, UTM Skudai, Johor Bahru, Johor, 81310
Ismail Ware
Metabolites Profiling Laboratory, Institute of Bioproduct Development
Email: chualeesuan@utm.my
Малайзия, UTM Skudai, Johor Bahru, Johor, 81310