Extraction and Determination of Two Antidepressant Drugs in Human Plasma by Dispersive Liquid–Liquid Microextraction‒HPLC
- Autores: Vaghar-Lahijani G.1, Saber-Tehrani M.1, Aberoomand-Azar P.1, Soleimani M.2
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Afiliações:
- Department of Chemistry, Science and Research Branch
- Department of Chemistry, Lahijan Branch
- Edição: Volume 73, Nº 2 (2018)
- Páginas: 145-151
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182967
- DOI: https://doi.org/10.1134/S1061934818020144
- ID: 182967
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Resumo
In this study, an effective method of ultrasound-assisted ionic liquid based dispersive liquid–liquid microextraction (UA−IL−DLLME) coupled with HPLC was applied for extraction and determination of two antidepressant drugs: venlafaxine hydrochloride and amitriptyline hydrochloride from human plasma samples. Three ionic liquids were studied: 1-butyl-3-methyl imidazolium hexafluorophosphate, 1-hexyl-3- methyl imidazolium hexa-fluoro-phosphate, and 1-octyl-3-methyl imidazolium hexafluorophosphate [C8MIM][PF6]. Various factors affect the stages and efficiency of extraction, some of which are pH of sample solution, type and volume of ionic liquid, the time of ultrasonication, centrifuging time and rate, and the ionic strength of solution. In this research, optimum conditions were obtained as 55 μL of [C8MIM][PF6] selected as ionic liquid, pH 11, 2% NaCl, 4 min ultrasonication and 5 min centrifuging at 3500 rpm. Under the optimized conditions, the linearity was obtained in the range of 0.2 to 250 μg/L. The limits of detection were 0.5 μg/L for venlafaxine and 0.8 μg/L for amitriptyline. Pre-concentration factors were 1.3 × 103 for venlafaxine and 1.2 × 103 for amitriptyline. The UA−IL−DLLME method coupled with HPLC was successfully used for the determination of venlafaxine and amitriptyline spiked into the real samples of human plasma.
Sobre autores
Gholamreza Vaghar-Lahijani
Department of Chemistry, Science and Research Branch
Email: Drmsabertehrani@yahoo.com
Irã, Tehran
Mohammad Saber-Tehrani
Department of Chemistry, Science and Research Branch
Autor responsável pela correspondência
Email: Drmsabertehrani@yahoo.com
Irã, Tehran
Parviz Aberoomand-Azar
Department of Chemistry, Science and Research Branch
Email: Drmsabertehrani@yahoo.com
Irã, Tehran
Mojtaba Soleimani
Department of Chemistry, Lahijan Branch
Email: Drmsabertehrani@yahoo.com
Irã, Lahijan