Chromatographic behavior of anthocyanins on a C10CN stationary phase
- Autores: Deineka V.1, Kul’chenko Y.1, Deineka L.1
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Afiliações:
- Institute of Engineering Technology and Natural Sciences
- Edição: Volume 72, Nº 12 (2017)
- Páginas: 1233-1238
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182690
- DOI: https://doi.org/10.1134/S106193481712005X
- ID: 182690
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Resumo
The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retention of anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.
Sobre autores
V. Deineka
Institute of Engineering Technology and Natural Sciences
Autor responsável pela correspondência
Email: deineka@bsu.edu.ru
Rússia, Belgorod, 308015
Ya. Kul’chenko
Institute of Engineering Technology and Natural Sciences
Email: deineka@bsu.edu.ru
Rússia, Belgorod, 308015
L. Deineka
Institute of Engineering Technology and Natural Sciences
Email: deineka@bsu.edu.ru
Rússia, Belgorod, 308015