Separation of β-blocker and amino acid enantiomers on a mixed chiral sorbent modified with macrocyclic antibiotics eremomycin and vancomycin
- Autores: Fedorova I.1, Shapovalova E.1, Shpigun O.1
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Afiliações:
- Department of Chemistry
- Edição: Volume 72, Nº 1 (2017)
- Páginas: 76-82
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182286
- DOI: https://doi.org/10.1134/S1061934817010075
- ID: 182286
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Resumo
A mixed chiral sorbent based on silica with immobilized macrocyclic antibiotics eremomycin and vancomycin was synthesized. A possibility of the separation of enantiomers of β-blockers (metoprolol, pindolol, alprenolol, oxprenolol, labetalol, and atenolol) and amino acids (tryptophan, phenylalanine, DOPA, methionine, and acetyl glutamic acid) on this chiral sorbent by HPLC was studied. The influence of the composition of the mobile phase (pH of buffer solution, its concentration, content of organic modifier, and its nature) on the retention times of β-blocker and amino acid enantiomers, selectivity, and resolution of peaks was studied. It was shown that the mixed chiral sorbent has enantioselectivity to both classes of compounds, while silica modified with vancomycin has no ability to the separation of enantiomers of non-derivatized amino acids, and silica modified with eremomycin has no ability to the separation of β-blocker enantiomers. High values of resolution for amino acids (max Rs > 4) and β-blockers (max Rs > 1) were obtained.
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Sobre autores
I. Fedorova
Department of Chemistry
Autor responsável pela correspondência
Email: irinafedorova89@gmail.com
Rússia, Moscow, 119991
E. Shapovalova
Department of Chemistry
Email: irinafedorova89@gmail.com
Rússia, Moscow, 119991
O. Shpigun
Department of Chemistry
Email: irinafedorova89@gmail.com
Rússia, Moscow, 119991