Use of functionalized nanoporous silica for the microextraction by packed sorbent of ellagic acid from fruit juice
- Autores: Rahimi M.1, Hashemi P.1, Badiei A.2, Safdarian M.1
-
Afiliações:
- Department of Chemistry
- School of Chemistry
- Edição: Volume 71, Nº 1 (2016)
- Páginas: 35-41
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181413
- DOI: https://doi.org/10.1134/S1061934816010068
- ID: 181413
Citar
Resumo
A semi-automated microextraction by packed sorbent (MEPS) method was developed for the HPLC determination of ellagic acid (EA) in juice samples. The procedure includes enrichment of the analyte on a nanoporous silica sorbent by the MEPS method using a laboratory-made programmable apparatus. Three functionalized SBA-15 nanoporous sorbents were evaluated for this purpose and 3-[bis(2-hydroxyethyl)amino]propyl-triethoxysilane (HPTES)-SBA-15 showed superior efficiency. Only 2 mg of the adsorbent packed in a syringe’s hub was sufficient for a quantitative recovery of EA. Different factors influencing the performance of the MEPS method such as the nature of desorption solvent, elution volume, pH, number of extraction cycles (draw–eject) and sample volume were carefully studied and optimized. Under the optimized conditions, a detection limit of 0.8 mg/L and a linear calibration curve with an R2 of 0.9995 were obtained for EA. The average recovery of the analyte for juice samples was 97.6% with a relative standard deviation of 4.4% for six replicates. The method was successfully applied to the HPLC determination of EA in pomegranate and grape juice samples.
Sobre autores
Marzieh Rahimi
Department of Chemistry
Email: payman_hashemi@yahoo.com
Irã, Teheran
Payman Hashemi
Department of Chemistry
Autor responsável pela correspondência
Email: payman_hashemi@yahoo.com
Irã, Teheran
Alireza Badiei
School of Chemistry
Email: payman_hashemi@yahoo.com
Irã, Teheran
Mehdi Safdarian
Department of Chemistry
Email: payman_hashemi@yahoo.com
Irã, Teheran