Determination of Phosphonylated Tyrosine as a Marker of Exposure to Nerve Agents in Dried Blood Plasma Spots by HPLC–HRMS/MS
- Авторлар: Koryagina N.1, Aliushina T.1, Karakashev G.1, Savel’eva E.1, Khlebnikova N.1, Radilov A.1
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Мекемелер:
- Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
- Шығарылым: Том 74, № 13 (2019)
- Беттер: 1341-1348
- Бөлім: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183371
- DOI: https://doi.org/10.1134/S1061934819130070
- ID: 183371
Дәйексөз келтіру
Аннотация
A procedure for the determination of phosphonylated tyrosine, a marker of exposure to organophosphorus nerve agents, in dried blood plasma spot (DBS) samples by means of liquid chromatography/tandem high-resolution mass spectrometry was developed. The DBS samples on Protein Saver 903 Card substrates were shown to be stable for two months on storage at 22 and 40°C. It was found that the temperature of storage has a high impact on the stability of the DBS samples: the analyte concentration in the samples stored at 40°C was about three times lower than that in the samples stored at room temperature. It was shown that the special Protein Saver 903 Card substrates could be replaced by an ordinary blue ribbon filter paper. In the latter case, the tyrosine adducts of nerve agents were reliably determined in the DBS samples stored for a month at room temperature (22°C). However, for storage at elevated temperatures (40°C), the use of filter paper substrates is not recommended. The proposed approach to storage of blood samples allows one to take and easily transport large series of samples and automate their analysis.
Авторлар туралы
N. Koryagina
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Хат алмасуға жауапты Автор.
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
T. Aliushina
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
G. Karakashev
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. Savel’eva
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
N. Khlebnikova
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
A. Radilov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical Biological Agency
Email: koryagina@rihophe.ru
Ресей, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663