Preconcentration of Catecholamins on Hypercrosslinked Polystyrene and Their Determination by High-Performance Liquid Chromatography
- Authors: Tolmacheva V.V.1,2, Yarykin D.I.1, Gorbunova M.V.1, Apyari V.V.1, Dmitrienko S.G.1, Zolotov Y.A.1,2
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Affiliations:
- Department of Chemistry, Moscow State University
- Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences
- Issue: Vol 74, No 11 (2019)
- Pages: 1057-1063
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183329
- DOI: https://doi.org/10.1134/S1061934819090107
- ID: 183329
Cite item
Abstract
The use of hypercrosslinked polystyrene for the group sorption preconcentration of catecholamines is proposed. The conditions for the preconcentration of noradrenaline, adrenaline, and dopamine are optimized: a microcolumn (10 × 6 mm), mass of adsorbent 0.03 g, 25 mL of solution (pH ~ 8.5), and transmission rate of the solution 1.0 mL/min. Compounds were desorbed with 1 mL of 6 M acetic acid and determined in the eluate by reversed-phase HPLC with an amperometric detector (E = 0.8 V). Adsorption preconcentration ensures the reduction of the limits of detection for catecholamines by more than 20 times. The limits of detection were 0.7 ng/mL for dopamine and 1 ng/mL for noradrenaline and adrenaline. The procedure was applied to the analysis of model mixtures based on urine.
About the authors
V. V. Tolmacheva
Department of Chemistry, Moscow State University; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991; Moscow, 119991
D. I. Yarykin
Department of Chemistry, Moscow State University
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
M. V. Gorbunova
Department of Chemistry, Moscow State University
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
V. V. Apyari
Department of Chemistry, Moscow State University
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
S. G. Dmitrienko
Department of Chemistry, Moscow State University
Author for correspondence.
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
Yu. A. Zolotov
Department of Chemistry, Moscow State University; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences
Email: dmitrienko@analyt.chem.msu.ru
Russian Federation, Moscow, 119991; Moscow, 119991