Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control
- Authors: Podolskiy I.I.1, Sobolevskii T.G.2, Dikunets M.A.1
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Affiliations:
- Antidoping Centre
- UCLA Olympic Analytical Laboratory
- Issue: Vol 73, No 3 (2018)
- Pages: 283-291
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182990
- DOI: https://doi.org/10.1134/S1061934818010082
- ID: 182990
Cite item
Abstract
The most difficult task in antidoping control is the identification of compounds whose origin can be either endogenous or exogenous. This paper presents a procedure for determining the origin of 19-norandrosterone based on the extraction of target components from urine, their multistage purification by semipreparative HPLC, acetylation, and quantification by gas chromatography–isotope-ratio mass spectrometry and gas chromatography–mass spectrometry. Effects of isotope fractionation were not observed in sample preparation. The degree of extraction of the target analytes was 80 ± 5%.
About the authors
I. I. Podolskiy
Antidoping Centre
Author for correspondence.
Email: podolskiy.i.i@gmail.com
Russian Federation, Moscow, 105005
T. G. Sobolevskii
UCLA Olympic Analytical Laboratory
Email: podolskiy.i.i@gmail.com
United States, Los Angeles, California, CA, 90025
M. A. Dikunets
Antidoping Centre
Email: podolskiy.i.i@gmail.com
Russian Federation, Moscow, 105005