Determination of Meldonium in human urine by HPLC with tandem mass spectrometric detection
- Authors: Azaryan A.A.1, Temerdashev A.Z.1, Dmitrieva E.V.1
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Affiliations:
- Kuban State University
- Issue: Vol 72, No 10 (2017)
- Pages: 1057-1060
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182646
- DOI: https://doi.org/10.1134/S1061934817100033
- ID: 182646
Cite item
Abstract
A procedure is proposed for determining Meldonium in human urine, including sample preparation to analysis and analyte determination by HPLC with tandem mass spectrometric detection. For sample preparation, the procedure of “dilute-and-shoot” was used. The lower limit of the analytical range is 10 ng/mL; the limit of detection is 7.5 ng/mL; and the linearity range is 10–250 ng/mL. The proposed procedure is tested on real samples obtained from volunteers. A possibility of the direct analysis of urine samples after dilution is demonstrated; the limit of detection is 20 ng/mL. The high sensitivity of the procedure ensures its use for the determination of Meldonium in clinical diagnosis and doping control.
Keywords
About the authors
A. A. Azaryan
Kuban State University
Author for correspondence.
Email: alice_azaryan@outlook.com
Russian Federation, Krasnodar, 350040
A. Z. Temerdashev
Kuban State University
Email: alice_azaryan@outlook.com
Russian Federation, Krasnodar, 350040
E. V. Dmitrieva
Kuban State University
Email: alice_azaryan@outlook.com
Russian Federation, Krasnodar, 350040