Central composite design optimization of dispersive liquid–liquid microextraction based on solidification of organic drop for the determination of 5-hydroxymethyl-2-furfural in orange juice using high-performance liquid chromatography
- Authors: Shiri F.1, Hashemi B.1, Sobhani S.1
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Affiliations:
- Department of Chemistry
- Issue: Vol 72, No 6 (2017)
- Pages: 671-677
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182514
- DOI: https://doi.org/10.1134/S1061934817060065
- ID: 182514
Cite item
Abstract
A simple, rapid and inexpensive dispersive liquid–liquid microextraction based on solidification of organic drop combined with HPLC was developed for the extraction and determination of trace levels of 5-hydroxymethyl-2-furfural in fruit juice. Effect of variables such as extracting and dispersive solvent volume and pH were investigated simultaneously using experimental design. Under the optimum conditions, the calibration graph was linear in the range of 1−200 μg/L with the detection limit of 0.3 μg/L. The optimized method revealed a good precision with relative standard deviation of 2.2%.The performance of the method was evaluated for extraction and determination of 5-hydroxymethyl-2-furfural in orange juice sample.
About the authors
Fereshteh Shiri
Department of Chemistry
Author for correspondence.
Email: Fereshteh.shiri@gmail.com
Iran, Islamic Republic of, Zabol, 98615-538
Beshare Hashemi
Department of Chemistry
Email: Fereshteh.shiri@gmail.com
Iran, Islamic Republic of, Kermanshah, 67149-67346
Sajad Sobhani
Department of Chemistry
Email: Fereshteh.shiri@gmail.com
Iran, Islamic Republic of, Zabol, 98615-538