Determination of unsaturated fatty acids with a migrating double bond in complex biological matrices by gas chromatography with flame ionization and mass spectrometry detection
- Authors: Ivankin A.N.1,2, Oliferenko G.L.1, Kulikovskii A.V.2, Chernukha I.M.2, Semenova A.A.2, Spiridonov K.I.2, Nasonova V.V.2
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Affiliations:
- Moscow State Forest University
- Gorbatov All-Russian Research Institute of Meat Industry
- Issue: Vol 71, No 11 (2016)
- Pages: 1131-1137
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182167
- DOI: https://doi.org/10.1134/S1061934816110046
- ID: 182167
Cite item
Abstract
We present the results of determination of unsaturated fatty acids (FAs) in foods of animal origin. It is shown that, in addition to the main component, monounsaturated cis-9-octadecenoic (oleic) acid, other fatty acids may be present in the composition of lipids, depending on the type of raw materials used to prepare the food product. The determination of these acids can significantly vary depending on the presence of impurities and assay conditions. It is demonstrated that gas chromatographic systems with a flame ionization detector, conventionally used for the determination of the fatty acid composition, are useless for the reliable identification of close structural analogues of unsaturated essential fatty acids. The conditions of determination of these compounds with mass spectrometric detection are selected. The effect of the composition of analytes, which were animal ingredients, on the results is discussed. It is shown that the greatest difficulty is the determination of unsaturated fatty acids present in test samples of different aetiologies in amounts not exceeding 0.05–0.1%.
About the authors
A. N. Ivankin
Moscow State Forest University; Gorbatov All-Russian Research Institute of Meat Industry
Author for correspondence.
Email: aivankin@mgul.ac.ru
Russian Federation, Mytishchi, Moscow oblast, 141005; Moscow, 109316
G. L. Oliferenko
Moscow State Forest University
Email: aivankin@mgul.ac.ru
Russian Federation, Mytishchi, Moscow oblast, 141005
A. V. Kulikovskii
Gorbatov All-Russian Research Institute of Meat Industry
Email: aivankin@mgul.ac.ru
Russian Federation, Moscow, 109316
I. M. Chernukha
Gorbatov All-Russian Research Institute of Meat Industry
Email: aivankin@mgul.ac.ru
Russian Federation, Moscow, 109316
A. A. Semenova
Gorbatov All-Russian Research Institute of Meat Industry
Email: aivankin@mgul.ac.ru
Russian Federation, Moscow, 109316
K. I. Spiridonov
Gorbatov All-Russian Research Institute of Meat Industry
Email: aivankin@mgul.ac.ru
Russian Federation, Moscow, 109316
V. V. Nasonova
Gorbatov All-Russian Research Institute of Meat Industry
Email: aivankin@mgul.ac.ru
Russian Federation, Moscow, 109316