Application of thin-layer chromatography image analysis technique in quantitative determination of sphingomyelin
- Authors: Skowron M.1, Zakrzewski R.1, Ciesielski W.1
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Affiliations:
- Faculty of Chemistry
- Issue: Vol 71, No 8 (2016)
- Pages: 808-813
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181971
- DOI: https://doi.org/10.1134/S1061934816080116
- ID: 181971
Cite item
Abstract
A sensitive and convenient method for sphingomyelin determination was developed based on thinlayer chromatography (TLC) and image-processing analysis. The mobile phase composition, detection and quantification conditions were systematically investigated through several trials. The molybdenum blue reaction allowed specific detection of the phospholipid with a high sensitivity and a wide linear range. Digital images of TLC plate chromatograms were captured with flatbed scanner and converted into peak chromatograms using TLSee® software and quantitative analysis was conducted. The linearity, sensitivity, accuracy and precision of the system were evaluated. The limits of detection and quantification were 0.5 and 1.7 μg/spot, respectively. Separation of the mixture consisting of sphingomyelin, phosphatidylcholine, and phosphatidylethanolamine was also carried out.
Keywords
About the authors
Monika Skowron
Faculty of Chemistry
Author for correspondence.
Email: mskowron@uni.lodz.pl
Poland, Tamka 12, Łódź, 91-403
Robert Zakrzewski
Faculty of Chemistry
Email: mskowron@uni.lodz.pl
Poland, Tamka 12, Łódź, 91-403
Witold Ciesielski
Faculty of Chemistry
Email: mskowron@uni.lodz.pl
Poland, Tamka 12, Łódź, 91-403