Investigation of electrochemical behavior and development of a validated adsorptive stripping square wave voltammetry method for 3-nitrotyrosine in human plasma and cerebrospinal fluid
- Authors: Acar E.T.1,2, Onar A.N.1, Onar M.K.3
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Affiliations:
- Faculty of Art and Science, Department of Analytical Chemistry 55139
- 26 August Campus, Faculty of Pharmacy, Department of Analytical Chemistry 34755
- Kurupelit Campus, Faculty of Medicine 55139
- Issue: Vol 71, No 6 (2016)
- Pages: 581-589
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181776
- DOI: https://doi.org/10.1134/S1061934816040031
- ID: 181776
Cite item
Abstract
An adsorptive stripping square wave voltammetric (AdSqW) method was developed for the determination of 3-nitrotyrosine (3-NT), a biomarker of in vivo oxidative damage in Alzheimer, ALS, Parkinson’s, cardiovascular diseases and cancer, in some biological fluids. Voltammetric measurements were performed in 0.30 M (pH 9.0) phosphate solution as supporting electrolyte, a reduction peak was observed at–0.487 V (vs. Ag/AgCl–3 M KCl) with a hanging mercury drop electrode by square wave voltammetry. Cyclic voltammetric measurements showed that the current was adsorption-controlled. LOD and LOQ values were as 0.25 and 1.5 nM, respectively, for the AdSqW method. 3-NT was determined in plasma and cerebrospinal fluid using AdSqW method, which allowed to work at low concentrations. Recovery value was measured as 96.3 ± 2.3%.
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About the authors
Ebru Türköz Acar
Faculty of Art and Science, Department of Analytical Chemistry 55139; 26 August Campus, Faculty of Pharmacy, Department of Analytical Chemistry 34755
Author for correspondence.
Email: ebruturkozacar@gmail.com
Turkey, Samsun; Istanbul
Atiye Nur Onar
Faculty of Art and Science, Department of Analytical Chemistry 55139
Email: ebruturkozacar@gmail.com
Turkey, Samsun
Musa Kazım Onar
Kurupelit Campus, Faculty of Medicine 55139
Email: ebruturkozacar@gmail.com
Turkey, Samsun