Optical assessment of Dunaliella salina microalgae viability upon toxicological testing
- Авторы: Chumakov D.S.1, Golubev A.A.1, Dykman L.A.1, Khlebtsov N.G.1, Bogatyrev V.A.1
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Учреждения:
- Institute of Biochemistry and Physiology of Plants and Microorganisms
- Выпуск: Том 79, № 6 (2017)
- Страницы: 844-848
- Раздел: Article
- URL: https://journals.rcsi.science/1061-933X/article/view/202399
- DOI: https://doi.org/10.1134/S1061933X17060059
- ID: 202399
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Аннотация
A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of an absorption peak at 680 nm corrected for the nonselective extinction as calculated using the values of nearest local minima at 640 and 740 nm.
Об авторах
D. Chumakov
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: bogatyrev_v@ibppm.ru
Россия, Saratov, 410049
A. Golubev
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: bogatyrev_v@ibppm.ru
Россия, Saratov, 410049
L. Dykman
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: bogatyrev_v@ibppm.ru
Россия, Saratov, 410049
N. Khlebtsov
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: bogatyrev_v@ibppm.ru
Россия, Saratov, 410049
V. Bogatyrev
Institute of Biochemistry and Physiology of Plants and Microorganisms
Автор, ответственный за переписку.
Email: bogatyrev_v@ibppm.ru
Россия, Saratov, 410049
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