Optical assessment of  Dunaliella salina  microalgae viability upon toxicological testing

D. S. Chumakov; A. A. Golubev; L. A. Dykman; N. G. Khlebtsov; V. A. Bogatyrev

doi:10.1134/S1061933X17060059

Optical assessment of Dunaliella salina microalgae viability upon toxicological testing

Autores: Chumakov D.S.¹, Golubev A.A.¹, Dykman L.A.¹, Khlebtsov N.G.¹, Bogatyrev V.A.¹
Afiliações:
1. Institute of Biochemistry and Physiology of Plants and Microorganisms
Edição: Volume 79, Nº 6 (2017)
Páginas: 844-848
Seção: Article
URL: https://journals.rcsi.science/1061-933X/article/view/202399
DOI: https://doi.org/10.1134/S1061933X17060059
ID: 202399

Citar

Texto integral

Acesso aberto
Acesso é fechado

Acesso está concedido
Acesso é fechado

Somente assinantes

Resumo
Sobre autores
Bibliografia
Arquivos suplementares
Estatísticas

Resumo

A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of an absorption peak at 680 nm corrected for the nonselective extinction as calculated using the values of nearest local minima at 640 and 740 nm.