Optical assessment of  Dunaliella salina  microalgae viability upon toxicological testing

D. S. Chumakov; A. A. Golubev; L. A. Dykman; N. G. Khlebtsov; V. A. Bogatyrev

doi:10.1134/S1061933X17060059

Optical assessment of Dunaliella salina microalgae viability upon toxicological testing

Authors: Chumakov D.S.¹, Golubev A.A.¹, Dykman L.A.¹, Khlebtsov N.G.¹, Bogatyrev V.A.¹
Affiliations:
1. Institute of Biochemistry and Physiology of Plants and Microorganisms
Issue: Vol 79, No 6 (2017)
Pages: 844-848
Section: Article
URL: https://journals.rcsi.science/1061-933X/article/view/202399
DOI: https://doi.org/10.1134/S1061933X17060059
ID: 202399

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of an absorption peak at 680 nm corrected for the nonselective extinction as calculated using the values of nearest local minima at 640 and 740 nm.