CD36 expression intensity on different subpopulations of circulating monocytes depending on the extent of subclinical atherosclerosis
- 作者: Dolgushin I.1, Genkel V.2, Baturina I.2, Savochkina A.2, Minasova A.2, Kuznetsova A.2, Shaposhnik I.2
-
隶属关系:
- South-Ural State Medical University
- South Ural State Medical University
- 期: 卷 24, 编号 4 (2021)
- 页面: 483-488
- 栏目: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/120174
- DOI: https://doi.org/10.46235/1028-7221-1046-CEI
- ID: 120174
如何引用文章
全文:
详细
It is known that chronic activation of innate immunity and persistent low-intensity inflammation play a crucial role in the initiation and progression of atherosclerosis. It was found that atherogenic lipoproteins can act as inducers of the inflammatory response through ligand-receptor interaction with pattern-recognizing receptors of immunocompetent cells, such as CD36 (SR-B2) and Toll-like receptors. It is suggested that expression of CD36 on circulating monocytes may represent the burden of systemic atherosclerosis and, therefore, act as its diagnostic marker. The aim of the present study was to assess the intensity of CD36 expression on circulating monocytes of different subpopulations in patients without established cardiovascular disease (CVD) depending on the extent of subclinical atherosclerosis of peripheral arteries. One hundred patients without established atherosclerotic CVD, 49 (49.0%) men and 51 (51.0%) women, were included in the study. Monoclonal antibody conjugates were used to phenotype monocyte subpopulations. The expression of CD36 on CD14++CD16- monocytes (classical monocytes), CD14+CD16+ monocytes (intermediate monocytes), CD14+CD16++ monocytes (non-classical monocytes) was determined by the average fluorescence intensity. There was a statistically significant decrease in CD36 expression intensity on classical and non-classical monocytes with increasing number of vascular basins affected by atherosclerosis. A statistically significant decrease in CD36 expression intensity on classical and non-classical monocytes was found in the patients with two vascular beds lesions in comparison with patients with a single vascular bed lesion upon pairwise comparisons.
作者简介
I. Dolgushin
South-Ural State Medical University
Email: dol-ii@mail.ru
ORCID iD: 0000-0002-0901-8042
PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, President, Head, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Director, Research Institute of Immunology
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦V. Genkel
South Ural State Medical University
编辑信件的主要联系方式.
Email: henkel-07@mail.ru
ORCID iD: 0000-0001-5902-3803
PhD (Medicine), Associate Professor, Department for Propaedeutics of Internal Diseases
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
Phone: 7 (951) 441-70-61
俄罗斯联邦I. Baturina
South Ural State Medical University
Email: irisha_baturina@mail.ru
ORCID iD: 0000-0002-5960-4189
PhD (Medicine), Senior Research Associate, Research Institute of Immunology
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦A. Savochkina
South Ural State Medical University
Email: alina7423@mail.ru
ORCID iD: 0000-0002-0536-0924
PhD, MD (Medicine), Professor, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Chief Research Associate, Research Institute of Immunology
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦A. Minasova
South Ural State Medical University
Email: pandora_anna@mail.ru
ORCID iD: 0000-0002-9084-0577
PhD (Biology), Associate Professor, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦A. Kuznetsova
South Ural State Medical University
Email: kuzja321@mail.ru
ORCID iD: 0000-0002-1136-7284
PhD (Medicine), Associate Professor, Department of Hospital Therapy
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦I. Shaposhnik
South Ural State Medical University
Email: shaposhnik@yandex.ru
ORCID iD: 0000-0002-7731-7730
MD, PhD (Medicine), Professor, Head, Department for Propaedeutics of Internal Diseases
454092, Russian Federation, Chelyabinsk, Vorovsky str., 64
俄罗斯联邦参考
- Гусев Е.Ю., Зотова Н.В., Журавлева Ю.А., Черешнев В.А. Физиологическая и патогенетическая роль рецепторов-мусорщиков у человека // Медицинская иммунология, 2020. Т. 22, № 1. С. 7-48. doi: 10.15789/1563-0625-PAP-1893.
- Chandran J., Wadhwa N., Madhu S.V., Kumar R., Sharma S. Monocyte CD36 expression associates with atherosclerotic burden in diabetes mellitus. Diabetes Res. Clin. Pract., 2020, Vol. 163, 108156. doi: 10.1016/j.diabres.2020.108156.
- Gómez-Bañuelos E., Martín-Márquez B.T., Martínez-García E.A., Figueroa-Sanchez M., NuñezAtahualpa L., Rocha-Muñoz A.D., Sánchez-Hernández P.E., Navarro-Hernandez R.E., Madrigal-Ruiz P.M., SaldañaMillan A.A., Duran-Barragan S., Gonzalez-Lopez L., Gamez-Nava J.I., Vázquez-Del Mercado M. Low levels of CD36 in peripheral blood monocytes in subclinical atherosclerosis in rheumatoid arthritis: a cross-sectional study in a Mexican population. Biomed Res. Int., 2014, Vol. 2014, 736786. doi: 10.1155/2014/736786.
- Liberale L., Montecucco F., Tardif J.C., Libby P., Camici G.G. Inflamm-ageing: the role of inflamemation in age-dependent cardiovascular disease. Eur. Heart J., 2020, Vol. 41, no. 31, pp. 2974-2982.
- Park Y.M. CD36, a scavenger receptor implicated in atherosclerosis. Exp. Mol. Med., 2014, Vol., 46, no. 6, e99. doi: 10.1038/emm.2014.38.
- Piechota M., Banaszewska A., Dudziak J., Slomczynski M., Plewa R. Highly upregulated expression of CD36 and MSR1 in circulating monocytes of patients with acute coronary syndromes. Protein J., 2012, Vol. 31, no. 6, pp. 511-518.
- Tian K., Xu Y., Sahebkar A., Xu S. CD36 in atherosclerosis: pathophysiological mechanisms and therapeutic implications. Curr. Atheroscler. Rep., 2020, Vol. 22, no. 10, 59. doi: 10.1007/s11883-020-00870-8.
- Yassin L.M., Londoño J., Montoya G., De Sanctis J.B., Rojas M., Ramírez L.A., García L.F., Vásquez G. Atherosclerosis development in SLE patients is not determined by monocytes ability to bind/endocytose Ox-LDL. Autoimmunity, 2011, Vol. 44, no. 3, pp. 201-210.
- Yazgan B., Sozen E., Karademir B., Ustunsoy S., Ince U., Zarkovic N., Ozer N.K. CD36 expression in peripheral blood mononuclear cells reflects the onset of atherosclerosis. Biofactors, 2018, Vol. 44, no. 6, pp. 588-596.
- Zhao L., Varghese Z., Moorhead J.F., Chen Y., Ruan X.Z. CD36 and lipid metabolism in the evolution of atherosclerosis. Br. Med. Bull., 2018, Vol. 126, no. 1, pp. 101-112
补充文件
