SOME BIOLOGICAL FEATURES OF ANTI-IDIOTIPIC AND TERTIARY ANTIBODIES AGAINST TWO MORPHINE DERIVATIVES
- 作者: Berzina A.1, Ulyanova L.1, Gamaleya N.1, Klimova T.1, Trofimov A.2, Stankova N.3
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隶属关系:
- V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
- The State Research Institute of Highly Pure Biopreparations, FMBA of Russia
- Scientific Center for Biomedical Technology, FMBA of Russia
- 期: 卷 22, 编号 2-1 (2019)
- 页面: 162-164
- 栏目: ORIGINAL ARTICLES
- URL: https://journals.rcsi.science/1028-7221/article/view/119443
- DOI: https://doi.org/10.31857/S102872210006436-4
- ID: 119443
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详细
The horse anti-idiotypic antibodies (Ab2) were obtained on the base of 2 mouse monoclonal antibodies (mAb) specific for two morphine derivatives – 3-0-carboxymethyl (CMM) and 6-hemisuccinyl (GSM). Immunization of rabbits and mini-pigs with Ab2 led to the formation of tertiary antibodies (Ab3). It was found that Ab2 antibodies have morphine-like properties, i. e. stimulate DNA synthesis in cultured human glioblastoma cells of the T98G line, which expresses a significant amount of opioid receptors on cell membrane. The addition of Ab3 antibodies to the System in vitro led to the abolition of the stimulating effect.
作者简介
A. Berzina
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
编辑信件的主要联系方式.
Email: berzina07@mail.ru
PhD, senior researcher,
Moscow
俄罗斯联邦L. Ulyanova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
Email: fake@neicon.ru
doctor of biological sciences, leading researcher,
Moscow
俄罗斯联邦N. Gamaleya
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
Email: fake@neicon.ru
doctor of medical sciences, professor, head of laboratory,
Moscow
俄罗斯联邦T. Klimova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
Email: fake@neicon.ru
PhD, senior researcher,
Moscow
俄罗斯联邦A. Trofimov
The State Research Institute of Highly Pure Biopreparations, FMBA of Russia
Email: fake@neicon.ru
group leader,
St Petersburg
俄罗斯联邦N. Stankova
Scientific Center for Biomedical Technology, FMBA of Russia
Email: fake@neicon.ru
PhD, head of laboratory,
Setllement Svetlye Gory
俄罗斯联邦参考
- Всемирный доклад о наркотиках. Управление Организации Объединенных Наций по наркотикам и преступности, 2015. Организация Объединенных Наций: Нью-Йорк. World Drug Report. United Nations Office on Drugs and Crime, 2015. United Nations: New York.
- Schabacker D. S., Kirschbaum K. S., Segre M. Exploring the feasibility of an anti-idiotypic cocaine vaccine: analysis of the specificity of anticocaine antibodies (Ab1) capable of inducing Ab2 anti-idiotypic antibodies. Immunology. 2000, 100, 48–56.
- Гамалея Н. Б., Берзина А. Г., Ульянова Л. И. Методологические основы создания вакцины для иммунотерапии зависимости от опиатов. Вопросы наркологии. 2017, № 4–5, 32–56.
- Кулаев Д. В., Насибов С. М., Маркин С. С., Бобков Ю. Г., Семенов М. П. Хроматографический способ выделения альфа-фетопротеина. Патент на изобретение RU № 2094078, 1997.
- Гамалея Н. Б., Ульянова М. А., Берзина А. Г., Ульянова Л. И. Тестирование психоактивных веществ и антител к ним в модели in vitro. Наркология. 2014, № 11, 37–41.
