SOME BIOLOGICAL FEATURES OF ANTI-IDIOTIPIC AND TERTIARY ANTIBODIES AGAINST TWO MORPHINE DERIVATIVES
- Authors: Berzina A.G.1, Ulyanova L.I.1, Gamaleya N.B.1, Klimova T.A.1, Trofimov A.V.2, Stankova N.V.3
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Affiliations:
- V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
- The State Research Institute of Highly Pure Biopreparations, FMBA of Russia
- Scientific Center for Biomedical Technology, FMBA of Russia
- Issue: Vol 22, No 2-1 (2019)
- Pages: 162-164
- Section: ORIGINAL ARTICLES
- URL: https://journals.rcsi.science/1028-7221/article/view/119443
- DOI: https://doi.org/10.31857/S102872210006436-4
- ID: 119443
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Abstract
The horse anti-idiotypic antibodies (Ab2) were obtained on the base of 2 mouse monoclonal antibodies (mAb) specific for two morphine derivatives – 3-0-carboxymethyl (CMM) and 6-hemisuccinyl (GSM). Immunization of rabbits and mini-pigs with Ab2 led to the formation of tertiary antibodies (Ab3). It was found that Ab2 antibodies have morphine-like properties, i. e. stimulate DNA synthesis in cultured human glioblastoma cells of the T98G line, which expresses a significant amount of opioid receptors on cell membrane. The addition of Ab3 antibodies to the System in vitro led to the abolition of the stimulating effect.
About the authors
A. G. Berzina
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
Author for correspondence.
Email: berzina07@mail.ru
PhD, senior researcher,
Moscow
Russian FederationL. I. Ulyanova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
doctor of biological sciences, leading researcher,
Moscow
Russian FederationN. B. Gamaleya
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
doctor of medical sciences, professor, head of laboratory,
Moscow
Russian FederationT. A. Klimova
V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of Russia
PhD, senior researcher,
Moscow
Russian FederationA. V. Trofimov
The State Research Institute of Highly Pure Biopreparations, FMBA of Russia
group leader,
St Petersburg
Russian FederationN. V. Stankova
Scientific Center for Biomedical Technology, FMBA of Russia
PhD, head of laboratory,
Setllement Svetlye Gory
Russian FederationReferences
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