ACTIVATION OF INTERFERON SYSTEM GENE TRANSRIPTION BY YEAST DOUBLE-STRANDED RNA

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In experiments on mice, the level of expression of antiviral-response-mediating proteins was studied in peritoneal macrophages, in reaction to the intraperitoneal administration of double-stranded RNA (dsRNA) from the yeast Saccharomyces cerevisiae. Dose (0.5 and 1.5 mg/kg) and time (5 and 24 hours) dependencies of the eff ect of dsRNA on the activity of genes encoding Toll-like receptor 3 (TLR3), IFN-alpha, IFN-beta, 2’,5’-oligoadenylatesynthetase (OAS) and proteinkinase R were determined. The obtained results indicate the increase in the level of transcriptional activity in the cells, most pronounced for TLR3, IFN-alpha and OAS genes. The maximum eff ect was observed 5 hours after the administration of dsRNA in the eff ective antiviral dose (0.5 mg / kg).

作者简介

A. Bateneva

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

编辑信件的主要联系方式.
Email: bateneva_av@vector.nsc.ru

Researcher, Department of Biological Research, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

俄罗斯联邦

S. Gamaley

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Email: fake@neicon.ru

Researcher, Department of Biological Research, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

俄罗斯联邦

L. Lebedev

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Email: fake@neicon.ru

Dr.habil., Head of the laboratory of Nucleic Acids and Recombinant Proteins, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

俄罗斯联邦

E. Danilenko

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Email: fake@neicon.ru

Dr.habil., Head of the laboratory of Nucleic Acids and Recombinant Proteins, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

俄罗斯联邦

参考

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  4. Соколова Т. М. Иммунное узнавание вирусных нуклеиновых кислот приводит к индукции интерферонов и воспалительных цитокинов. В сб. науч. статей «Интерферон-2011», М. 2012, 52–63
  5. Williams B., Gilbert C., Kerr I. The respective roles of the protein kinase and pppA2’ p5’ A2’ p5’ A-activated endonuclease in the inhibition of protein synthesis by double stranded RNA in rabbit reticulocyte lysates. Nucleic Acids Research. 1979, 6(4), 1335–1350.

版权所有 © Bateneva A.V., Gamaley S.G., Lebedev L.R., Danilenko E.D., 2019

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