ACTIVATION OF INTERFERON SYSTEM GENE TRANSRIPTION BY YEAST DOUBLE-STRANDED RNA

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Abstract

In experiments on mice, the level of expression of antiviral-response-mediating proteins was studied in peritoneal macrophages, in reaction to the intraperitoneal administration of double-stranded RNA (dsRNA) from the yeast Saccharomyces cerevisiae. Dose (0.5 and 1.5 mg/kg) and time (5 and 24 hours) dependencies of the eff ect of dsRNA on the activity of genes encoding Toll-like receptor 3 (TLR3), IFN-alpha, IFN-beta, 2’,5’-oligoadenylatesynthetase (OAS) and proteinkinase R were determined. The obtained results indicate the increase in the level of transcriptional activity in the cells, most pronounced for TLR3, IFN-alpha and OAS genes. The maximum eff ect was observed 5 hours after the administration of dsRNA in the eff ective antiviral dose (0.5 mg / kg).

About the authors

A. V. Bateneva

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Author for correspondence.
Email: bateneva_av@vector.nsc.ru

Researcher, Department of Biological Research, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

Russian Federation

S. G. Gamaley

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Researcher, Department of Biological Research, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

Russian Federation

L. R. Lebedev

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Dr.habil., Head of the laboratory of Nucleic Acids and Recombinant Proteins, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

Russian Federation

E. D. Danilenko

Institute of Medical Biotechnology FBRI SRC VB “Vector” Rospotrebnadzor

Dr.habil., Head of the laboratory of Nucleic Acids and Recombinant Proteins, Institute of Medical Biotechnology,

Berdsk, Novosibirsk Region

Russian Federation

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Copyright (c) 2019 Bateneva A.V., Gamaley S.G., Lebedev L.R., Danilenko E.D.

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