Activity of cellular immunity in patients with nutrition-dependent diseases (obesity, type 2 diabetes)

Cover Page

Cite item

Full Text

Abstract

The purpose of the study is to analyze the indicators of cellular immunity in patients with obesity (OB) and type 2 diabetes mellitus (DM2). The study included 15 patients with DM2 (DM2 group), body mass index (BMI) 37.36±1.12 kg/m2, 12 patients with OB (OB group), BMI – 38.12±1.51 kg/m2 and 15 practically healthy individuals (comparison group – GС): BMI 23.5±0.15 kg/m2. The study was performed on a flow cytometer FC-500 (Beckman Coulter, USA) using double combinations of monoclonal antibodies (Beckman Coulter – Immunotech SAS, France). The percentage indicators of the T cell population were assessed, such as total number of T lymphocytes (CD3+), number of T helper cells (CD3+CD4+), cytotoxic T lymphocytes (CD3+CD8+), natural killer cells – NK cells (CD3-CD16+CD56+), natural killer cells with the properties of T lymphocytes – NKT cells (CD3+CD16+CD56+) and B cell population (CD19+) lymphocytes, as well as the relative content of lymphocytes carrying activation markers (CD3+HLA-DR+, CD3+CD25+), and apoptosis marker antigen CD45+CD95+. The relative content of B lymphocytes and T lymphocytes in the peripheral blood of the examined individuals did not have statistically significant differences. In patients in the DM2 and OB groups, there was a tendency (p < 0.1) towards an increase in the relative content of T helper cells and a significant (p < 0.05) decrease in the percentage of T cytotoxic lymphocytes relative to GC. This redistribution of lymphocyte subpopulations led to a significant (p < 0.05) increase in the IRI value (arbitrary units): DM2 – 2.87±0.58; OB – 2.30±0.33 vs GC – 1.62±0.15. The relative content of NK cells and NKT cells in the peripheral blood of the examined individuals did not have a statistically significant difference in the magnitude of the indicators. A statistically significant (p < 0.05) increase in the relative content of T lymphocytes expressing the activation marker HLA-DR (CD3+HLA-DR+) was found in patients in the DM2 and OB groups relative to GC [(%) DM2 – 7.95±0, 81; OB – 6.54±0.24; GC – 4.01±0.91] and a significant (p < 0.05) increase in the percentage of CD45+CD95+ lymphocytes in patients with OB and DM2 relative to GC [(%) DM2 – 5.84±0.68; OB – 5.16±0.89; GC – 2.78±0.34]. The results obtained indicate the presence of meta-inflammation in patients with DM2 and OB.

About the authors

E. N. Trushina

Federal Research Centre of Nutrition, Biotechnology and Food Safety

Author for correspondence.
Email: trushina@ion.ru

PhD (Medicine), Head, Laboratory of Immunology

Russian Federation, Moscow

O. K. Mustafina

Federal Research Centre of Nutrition, Biotechnology and Food Safety

Email: trushina@ion.ru

PhD (Medicine), Senior Research Associate, Laboratory of Immunology

Russian Federation, Moscow

Kh. Kh. Sharafetdinov

Federal Research Centre of Nutrition, Biotechnology and Food Safety; Russian Medical Academy of Continuing Professional Education; I. Sechenov First Moscow State Medical University (Sechenov University)

Email: trushina@ion.ru

PhD, MD (Medicine), Head, Department of Metabolic Diseases and Diet Therapy of the Clinical Nutrition Clinic; Professor, Department of Dietetics and Nutritionology; Professor, Department of Food Hygiene and Toxicology

Russian Federation, Moscow; Moscow; Moscow

References

  1. Трушина Э.Н., Выборнов В.Д., Ригер Н.А., Мустафина О.К., Солнцева Т.Н., Тимонин А.Н., Зилова И.С., Раджабкадиев Р.М. Иммуномодулирующие эффекты использования L-карнитина и коэнзима Q10 в питании спортсменов-юниоров // Вопросы питания. 2019. Т. 88, № 2. С. 40-49. [Trushina E.N., Vybornov V.D., Riger N.A., Mustafina O.K., Solntseva T.N., Timonin A.N., Zilova I.S., Radzhabkadiev R.M. Immunomodulatory effects of using L-carnitine and coenzyme Q10 in the nutrition of junior athletes. Voprosy pitaniia = Problems of Nutrition, 2019, Vol. 88, no. 2. pp. 40-49. (In Russ.)]
  2. Ярец Ю.И. Интерпретация результатов иммунограммы. Гомель: ГУ «Республиканский научно-практический центр радиационной медицины и экологии человека», 2020. 38 с. [Yarets Yu.I. Interpretation of immunogram results]. Gomel: Republican Scientific and Practical Center for Radiation Medicine and Human Ecology, 2020. 38 p.
  3. Brunt E.M., Wong V.W., Nobili V., Day C.P., Sookoian S., Maher J.J., Bugianesi E., Sirlin Cl., Neuschwander-Tetri Br. A., Rinella M.E. Nonalcoholic fatty liver disease. Nat. Rev. Dis. Primers, 2015, no. 1, 15080. doi: 10.1038/nrdp.2015.80.
  4. Cable J., Rathmell J.C., Pearce E.L., Ho P.C., Haigis M.C., Mamedov M.R., Wu M.J., Kaech S.M., Lynch L., Febbraio M.A., Bapat S.P., Hong H.S., Zou W., Belkaid Y., Sullivan Z.A., Keller A., Wculek St.K.,. Green D.R., Postic C., Amit I., Benitah S.A., Jones R.G., Reina-Campos M., Torres S.V., Beyaz S., Brennan D., O’Neill L.A.J., Perry R.J., Brenner D. Immunometabolism at the crossroads of obesity and cancer-a Keystone Symposia report. Ann. N. Y. Acad. Sci., 2023, Vol. 1523, no. 1, pp. 38-50.
  5. Dunbar C.L., Aukema H.M., Calder P.C., Gibson D.L., Henrickson S.E., Khan S., Mailhot G., Panahi S., Tabung F.K., Tom M., Upton J.E.M., Winer D.A., Field C.J. Nutrition and immunity: perspectives on key issues and next steps. Appl. Physiol. Nutr. Metab., 2023, Vol. 48, no. 7, pp. 484-497.
  6. Eljaafari A., Pestel J., Le Magueresse-Battistoni B., Chanon S., Watson J., Robert M., Disse E., Vidal H. Adipose-tissue-derived mesenchymal stem cells mediate PD-L1 overexpression in the white adipose tissue of obese individuals, resulting in T cell dysfunction. Cells, 2021, Vol. 10, no. 10, 2645. doi: 10.3390/cells10102645.
  7. Granville D.J., Carthy C.M., Hunt D.W., McManus B.M. Apoptosis: Molecular aspects of cell death and disease. Lab. Investig., 1998, Vol. 78, no. 8, pp. 893-913.
  8. Kawai T., Autieri M.V., Scalia R. Adipose tissue inflammation and metabolic dysfunction in obesity. Am. J. Physiol. Cell Physiol., 2021, Vol. 320, no. 3, pp. 375-391.
  9. Li Y., Wang F., Imani S., Tao L., Deng Y., Cai Y. Natural killer cells: friend or foe in metabolic diseases? Front. Immunol., 2021, no. 12, 614429. doi: 10.3389/fimmu.2021.614429.
  10. Makowski L., Chaib M., Rathmell J.C. Immunometabolism: From basic mechanisms to translation. Immunol. Rev., 2020, Vol. 295, no. 1, pp. 5-14.
  11. Ortega F.B., Lavie C.J., Blair S.N. Obesity and cardiovascular disease. Circ. Res., 2016, Vol. 118, no. 11, pp. 1752-1770.
  12. Rivera-Carranza T., Nájera-Medina O., Bojalil-Parra R., Rodríguez-López C., Zúñiga-León E., León-Téllez Girón A., Azaola-Espinosa A. The link between lymphocyte subpopulations in peripheral blood and metabolic variables in patients with severe obesity. Peer J., 2023, no. 11, e15465. doi: 10.7717/peerj.15465.
  13. Wu H., Ballantyne C.M. Metabolic inflammation and insulin resistance in obesity. Circ. Res., 2020, Vol. 126, no. 11, pp. 1549-1564.
  14. Xu H., Chen Y., Li Y., Xia F., Han B., Zhang H., Zhai H., Wu H., Li Y., Lu Y. Mitochondrial apoptosis of lymphocyte is induced in type 2 diabetes. Chin. Med. J. (Engl), 2014, Vol. 127, no. 2, pp. 213-217.
  15. Zatterale F., Longo M., Naderi J., Raciti G.A., Desiderio A., Miele C., Beguinot F. Chronic adipose tissue inflammation linking obesity to insulin resistance and type 2 diabetes. Front. Physiol., 2020, no. 10, 1607. doi: 10.3389/fphys.2019.01607.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Figure 1. Relative content of lymphocyte subpopulations in the peripheral blood of patients with DM2, OB and GC

Download (88KB)
Indexing metadata
3. Figure 2. Expression of activation markers on lymphocytes in the peripheral blood of patients with DM2, OB and GC

Download (89KB)
Indexing metadata

Copyright (c) 2024 Trushina E.N., Mustafina O.K., Sharafetdinov K.K.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies