Dr. N.A. Vyrubov. About the degeneration of nerve cells and fibers in the spinal cord with growing paralytic dementia — Diss. SPb. 1899

The work was carried out under the guidance of prof. V. M. Bekhterev. The material for the study was 12 cases collected by the author in the hospital of St. Panteleimon and in the House of the Priesthood of the mentally ill Emperor Alexander III. When studying, the author simultaneously used various methods of painting. Staining of nerve cells was carried out according to Nissl's method and according to the method proposed by Weigert for coloring coriokinetic figures; for the simultaneous coloring of cells and fibers, medium and oxalic acid carmine, hematoxylin with eosin, van-Gieson's method were used; Weigert-Pal and Marchi methods were used for dyeing pulp fibers; neuroglia was stained according to the method of Weigert'a, Kulchitsky and Rehm'a.