Effects of stearic acid on the cryotolerance of the domestic cat (Felis silvestris catus) embryos
- Authors: Brusentsev E.Y.1, Okotrub S.V.1, Lebedeva D.A.1,2,3, Okotrub K.A.2, Rakhmanova T.A.1,2,3, Amstislavsky1 S.Y.1,2
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Affiliations:
- Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
- Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences
- Novosibirsk State University
- Issue: No 1 (2024)
- Pages: 76-89
- Section: ZOOLOGY
- URL: https://journals.rcsi.science/1026-3470/article/view/255519
- DOI: https://doi.org/10.31857/S1026347024010083
- EDN: https://elibrary.ru/LPLLRN
- ID: 255519
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Abstract
The current work aimed to study the effect of domestic cat (Felis silvestris catus) embryos in vitro exposure to saturated stearic acid (SA) and to evaluate how the change in lipid content affects the cryopreservation results. The addition of SA to the culture medium did not affect the development of cat embryos in vitro before cryopreservation. The total lipid amount in the SA-treated embryos was not changed as well. However, the lipid unsaturation degree was lower in embryos after in vitro exposure to SA. Moreover, the lipid phase transition onset temperature (T*) was higher in SA-treated embryos as compared with controls. These changes of intracellular lipids unsaturation degree and T* were associated with the impairment of embryo cryopreservation effectiveness. The results obtained may be of importance for the applying Genome Resource Banking concept to the Felinae species.
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About the authors
E. Yu. Brusentsev
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Author for correspondence.
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090
S. V. Okotrub
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090
D. A. Lebedeva
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090; Pirogova 2, Novosibirsk, 630090
K. A. Okotrub
Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, prosp. Koptyuga 1, Novosibirsk, 630090
T. A. Rakhmanova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090; Pirogova 2, Novosibirsk, 630090
S. Ya. Amstislavsky1
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090
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